EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.3.1.2 | - |
Citrobacter amalonaticus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.3.1.2 | 2,2'-dipyridyl | 70% loss of activity in the deamination reaction of L-threo-3-methylaspartate | Citrobacter amalonaticus | |
4.3.1.2 | 5,5'-dithiobis(2-nitrobenzoate) | complete inhibition in the deamination reaction | Citrobacter amalonaticus | |
4.3.1.2 | 8-hydroxyquinoline | 58% loss of activity in the deamination reaction of L-threo-3-methylaspartate | Citrobacter amalonaticus | |
4.3.1.2 | Ag+ | complete | Citrobacter amalonaticus | |
4.3.1.2 | Ca2+ | 55% loss of activity in the deamination reaction of L-threo-3-methylaspartate | Citrobacter amalonaticus | |
4.3.1.2 | Ca2+ | - |
Clostridium tetanomorphum | |
4.3.1.2 | Cd2+ | complete inhibition in the deamination reaction with L-threo-3-methylaspartate | Citrobacter amalonaticus | |
4.3.1.2 | Co2+ | 58% loss of activity in the deamination reaction of L-threo-3-methylaspartate | Citrobacter amalonaticus | |
4.3.1.2 | Cu2+ | 50% loss of activity in the deamination reaction of L-threo-3-methylaspartate | Citrobacter amalonaticus | |
4.3.1.2 | Cu2+ | - |
Clostridium tetanomorphum | |
4.3.1.2 | Fe2+ | 43% loss of activity in the deamination reaction of L-threo-3-methylaspartate | Citrobacter amalonaticus | |
4.3.1.2 | Hg+ | complete | Citrobacter amalonaticus | |
4.3.1.2 | Hg2+ | complete | Citrobacter amalonaticus | |
4.3.1.2 | hydroxylamine | - |
Clostridium tetanomorphum | |
4.3.1.2 | additional information | - |
Citrobacter amalonaticus | |
4.3.1.2 | N-ethylmaleimide | 36% loss of activity in the deamination raction of L-threo-3-methylaspartate | Citrobacter amalonaticus | |
4.3.1.2 | N-ethylmaleimide | - |
Clostridium tetanomorphum | |
4.3.1.2 | o-phenanthroline | 24% loss of activity in the deamination reaction of L-threo-3-methylaspartate | Citrobacter amalonaticus | |
4.3.1.2 | p-chloromercuribenzoate | complete | Citrobacter amalonaticus | |
4.3.1.2 | phenylhydrazine | - |
Clostridium tetanomorphum | |
4.3.1.2 | Sn2+ | 34% loss of activity in the deamination reaction of L-threo-3-methylaspartate | Citrobacter amalonaticus | |
4.3.1.2 | Zn2+ | 30% loss of activity in the deamination reaction of L-threo-3-methylaspartate | Citrobacter amalonaticus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.3.1.2 | Co2+ | activates | Clostridium tetanomorphum | |
4.3.1.2 | Co2+ | activates | Citrobacter amalonaticus | |
4.3.1.2 | Cs+ | required | Citrobacter amalonaticus | |
4.3.1.2 | K+ | - |
Clostridium tetanomorphum | |
4.3.1.2 | K+ | - |
Citrobacter amalonaticus | |
4.3.1.2 | Li+ | required | Citrobacter amalonaticus | |
4.3.1.2 | Mg2+ | - |
Clostridium tetanomorphum | |
4.3.1.2 | Mg2+ | - |
Citrobacter amalonaticus | |
4.3.1.2 | Mn2+ | activates | Clostridium tetanomorphum | |
4.3.1.2 | Mn2+ | activates | Citrobacter amalonaticus | |
4.3.1.2 | additional information | - |
Clostridium tetanomorphum | |
4.3.1.2 | additional information | - |
Citrobacter amalonaticus | |
4.3.1.2 | Na+ | required | Citrobacter amalonaticus | |
4.3.1.2 | NH4+ | required | Citrobacter amalonaticus | |
4.3.1.2 | Ni2+ | activates | Clostridium tetanomorphum | |
4.3.1.2 | Ni2+ | activates | Citrobacter amalonaticus | |
4.3.1.2 | Rb+ | required | Citrobacter amalonaticus | |
4.3.1.2 | Zn2+ | activates | Clostridium tetanomorphum | |
4.3.1.2 | Zn2+ | activates | Citrobacter amalonaticus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.3.1.2 | 42000 | - |
2 * 42000, SDS-PAGE | Citrobacter amalonaticus |
4.3.1.2 | 84000 | - |
gel filtration | Citrobacter amalonaticus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.1.2 | L-threo-3-methylaspartate | Clostridium tetanomorphum | - |
? | - |
? | |
4.3.1.2 | L-threo-3-methylaspartate | Citrobacter amalonaticus | - |
? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.3.1.2 | Citrobacter amalonaticus | - |
YG-1002 | - |
4.3.1.2 | Citrobacter amalonaticus YG-1002 | - |
YG-1002 | - |
4.3.1.2 | Clostridium tetanomorphum | - |
- |
- |
4.3.1.2 | Clostridium tetanomorphum | - |
NCIMB 11547 | - |
4.3.1.2 | Clostridium tetanomorphum H1 / ATCC 15920 | - |
- |
- |
4.3.1.2 | Clostridium tetanomorphum NCIMB 11547 | - |
NCIMB 11547 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.3.1.2 | - |
Citrobacter amalonaticus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.3.1.2 | additional information | - |
- |
Clostridium tetanomorphum |
4.3.1.2 | additional information | - |
- |
Citrobacter amalonaticus |
4.3.1.2 | 179 | - |
- |
Citrobacter amalonaticus |
4.3.1.2 | 282 | - |
incubation with 5 mM EDTA, pH 7.0, 4¦C, for 3 h | Citrobacter amalonaticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.1.2 | (2S,3R)-3-ethylaspartic acid | - |
Clostridium tetanomorphum | ? | - |
? | |
4.3.1.2 | (2S,3R)-3-ethylaspartic acid | - |
Citrobacter amalonaticus | ? | - |
? | |
4.3.1.2 | (2S,3S)-3-ethylaspartic acid | - |
Clostridium tetanomorphum | ? | - |
? | |
4.3.1.2 | (2S,3S)-3-ethylaspartic acid | - |
Citrobacter amalonaticus | ? | - |
? | |
4.3.1.2 | 3-chloroaspartate | - |
Clostridium tetanomorphum | chlorofumarate + NH3 | - |
? | |
4.3.1.2 | 3-chloroaspartate | (2R,3S)-3-chloroaspartic acid | Citrobacter amalonaticus | chlorofumarate + NH3 | - |
? | |
4.3.1.2 | 3-chloroaspartate | (2R,3S)-3-chloroaspartic acid | Citrobacter amalonaticus YG-1002 | chlorofumarate + NH3 | - |
? | |
4.3.1.2 | 3-chloroaspartate | - |
Clostridium tetanomorphum H1 / ATCC 15920 | chlorofumarate + NH3 | - |
? | |
4.3.1.2 | 3-chloroaspartate | - |
Clostridium tetanomorphum NCIMB 11547 | chlorofumarate + NH3 | - |
? | |
4.3.1.2 | ethylfumarate + NH3 | - |
Clostridium tetanomorphum | ? | - |
? | |
4.3.1.2 | ethylfumarate + NH3 | - |
Citrobacter amalonaticus | ? | - |
? | |
4.3.1.2 | ethylfumarate + NH3 | - |
Citrobacter amalonaticus YG-1002 | ? | - |
? | |
4.3.1.2 | ethylfumarate + NH3 | - |
Clostridium tetanomorphum H1 / ATCC 15920 | ? | - |
? | |
4.3.1.2 | ethylfumarate + NH3 | - |
Clostridium tetanomorphum NCIMB 11547 | ? | - |
? | |
4.3.1.2 | L-threo-3-methylaspartate | r | Clostridium tetanomorphum | mesaconate + NH3 | - |
? | |
4.3.1.2 | L-threo-3-methylaspartate | r | Citrobacter amalonaticus | mesaconate + NH3 | - |
? | |
4.3.1.2 | L-threo-3-methylaspartate | (2S,3S)-3-methylaspartic acid | Clostridium tetanomorphum | mesaconate + NH3 | - |
? | |
4.3.1.2 | L-threo-3-methylaspartate | (2S,3S)-3-methylaspartic acid | Citrobacter amalonaticus | mesaconate + NH3 | - |
? | |
4.3.1.2 | L-threo-3-methylaspartate | r | Citrobacter amalonaticus YG-1002 | mesaconate + NH3 | - |
? | |
4.3.1.2 | L-threo-3-methylaspartate | (2S,3S)-3-methylaspartic acid | Citrobacter amalonaticus YG-1002 | mesaconate + NH3 | - |
? | |
4.3.1.2 | L-threo-3-methylaspartate | r | Clostridium tetanomorphum H1 / ATCC 15920 | mesaconate + NH3 | - |
? | |
4.3.1.2 | L-threo-3-methylaspartate | (2S,3S)-3-methylaspartic acid | Clostridium tetanomorphum H1 / ATCC 15920 | mesaconate + NH3 | - |
? | |
4.3.1.2 | L-threo-3-methylaspartate | r | Clostridium tetanomorphum NCIMB 11547 | mesaconate + NH3 | - |
? | |
4.3.1.2 | L-threo-3-methylaspartate | (2S,3S)-3-methylaspartic acid | Clostridium tetanomorphum NCIMB 11547 | mesaconate + NH3 | - |
? | |
4.3.1.2 | L-threo-3-methylaspartate | - |
Clostridium tetanomorphum | ? | - |
? | |
4.3.1.2 | L-threo-3-methylaspartate | - |
Citrobacter amalonaticus | ? | - |
? | |
4.3.1.2 | additional information | - |
Clostridium tetanomorphum | ? | - |
? | |
4.3.1.2 | additional information | - |
Citrobacter amalonaticus | ? | - |
? | |
4.3.1.2 | additional information | - |
Citrobacter amalonaticus YG-1002 | ? | - |
? | |
4.3.1.2 | additional information | - |
Clostridium tetanomorphum H1 / ATCC 15920 | ? | - |
? | |
4.3.1.2 | additional information | - |
Clostridium tetanomorphum NCIMB 11547 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.3.1.2 | dimer | - |
Clostridium tetanomorphum |
4.3.1.2 | dimer | 2 * 42000, SDS-PAGE | Citrobacter amalonaticus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.3.1.2 | 50 | - |
maximal activity in the deamination reaction of L-threo-3-methylaspartate | Citrobacter amalonaticus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.3.1.2 | 45 | - |
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, 6% loss of activity | Citrobacter amalonaticus |
4.3.1.2 | 50 | - |
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, 10% loss of activity | Citrobacter amalonaticus |
4.3.1.2 | 55 | - |
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methylaspartate, 44% loss of activity | Citrobacter amalonaticus |
4.3.1.2 | 60 | - |
0.1 mM potassium phosphate buffer, pH 7.0, 10 min, in the deamination reaction of (2S,3S)-3-methxylaspartate, 96.5% loss of activity | Citrobacter amalonaticus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.3.1.2 | 8.5 | - |
maximal activity in TRIS/HCl buffer in the amination reaction of mesaconate | Citrobacter amalonaticus |
4.3.1.2 | 9.7 | - |
maximal activity in ethanolamine/HCl buffer in the deamination reaction of L-threo-3-methylaspartate | Citrobacter amalonaticus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
4.3.1.2 | additional information | - |
- |
Citrobacter amalonaticus |
4.3.1.2 | 5.5 | 11 | 30¦C, 10 min, deamination reaction of L-threo-3-methylaspartate, 14% loss of activity | Citrobacter amalonaticus |