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Literature summary extracted from

  • Paulsen, J.; Hustedt, H.
    Extractive purification of aspartase from Escherichia coli K12 (1994), Methods Enzymol., 228, 590-599.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.3.1.1 synthesis enzyme or whole cells are used for the industrial production of L-aspartate, which together with L-phenylalanine is the basis of the sweetener aspartame Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.1 Escherichia coli
-
 K12 strain JRG 1849
-
4.3.1.1 Pseudomonas fluorescens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.1
-
 Escherichia coli

Renatured (Commentary)

EC Number Renatured (Comment) Organism
4.3.1.1 renaturation by dialysis against 50 mM Tris-HCl buffer ,pH 8.0, at 4C for 2 days Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.1 additional information
-
-
 Escherichia coli
4.3.1.1 219
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
4.3.1.1 -20°C, stable for several months Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.1 L-aspartate r Escherichia coli fumarate + NH3
-
 ?
4.3.1.1 L-aspartate r Pseudomonas fluorescens fumarate + NH3
-
 ?
4.3.1.1 L-aspartate natural substrate Escherichia coli fumarate + NH3
-
 ?
4.3.1.1 L-aspartate natural substrate Pseudomonas fluorescens fumarate + NH3
-
 ?