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Literature summary extracted from
- alpha-1,4-Glucan lyase, a new class of starch/glycogen degrading enzyme. I. Efficient purification and characterization from red seaweeds (1993), Biochim. Biophys. Acta, 1156, 313-320.
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.13 | 98000 | - |
gel filtration | Gracilariopsis lemaneiformis |
| 4.2.2.13 | 111000 | - |
1 * 111000, SDS-PAGE | Gracilariopsis lemaneiformis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.2.13 | Gracilaria gracilis | - |
- |
- |
| 4.2.2.13 | Gracilariopsis lemaneiformis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.2.13 | - |
Gracilariopsis lemaneiformis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.13 | 93.3 | - |
- |
Gracilariopsis lemaneiformis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.2.13 | Amylopectin | - |
Gracilariopsis lemaneiformis | Glucose + 1,5-anhydro-D-fructose | - |
? |  |
| 4.2.2.13 | Glycogen | - |
Gracilariopsis lemaneiformis | ? | - |
? | |
| 4.2.2.13 | Maltoheptaose | - |
Gracilariopsis lemaneiformis | ? | - |
? | |
| 4.2.2.13 | Maltose | - |
Gracilariopsis lemaneiformis | Glucose + 1,5-anhydro-D-fructose | - |
? | |
| 4.2.2.13 | Maltotetraose | - |
Gracilariopsis lemaneiformis | ? | - |
? | |
| 4.2.2.13 | Pullulan | - |
Gracilariopsis lemaneiformis | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.2.13 | monomer | 1 * 111000, SDS-PAGE | Gracilariopsis lemaneiformis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.13 | 50 | - |
maltose or amylopectin as substrate | Gracilariopsis lemaneiformis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.13 | 33 | 65 | about 50% of maximal activity at 33°C and 65°C | Gracilariopsis lemaneiformis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.13 | 70 | - |
in absence of substrate, complete loss of activity after 5 min | Gracilariopsis lemaneiformis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.13 | 2.5 | 7 | maltose as substrate | Gracilariopsis lemaneiformis |
| 4.2.2.13 | 3.5 | 7.5 | amylopectin as substrate | Gracilariopsis lemaneiformis |
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Release 2023.1 (January 2023)
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