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Literature summary extracted from
- Isolation of 2-methylisocitrate dehydratase, a new enzyme serving in the methylcitric acid cycle for propionate metabolism, from Yallowia lipolytica (1995), Biosci. Biotechnol. Biochem., 59, 1825-1828.
No PubMed abstract available
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.99 | 0.018 | - |
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate | - |
Yarrowia lipolytica |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.99 | 69000 | - |
gel filtration | Yarrowia lipolytica |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.99 | (2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate | Yarrowia lipolytica | the enzyme is involved in methylcitric acid cycle for propionate metabolism | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.99 | Yarrowia lipolytica | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.99 | - |
Yarrowia lipolytica |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.99 | 1.33 | - |
- |
Yarrowia lipolytica |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.99 | (2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate | r | Yarrowia lipolytica | (Z)-But-2-ene-1,2,3-tricarboxylate + H2O | - |
? | |
| 4.2.1.99 | (2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate | no activity with: 2-methylcitrate, citrate, cis-aconitate, trans-aconitate, isocitrate | Yarrowia lipolytica | (Z)-But-2-ene-1,2,3-tricarboxylate + H2O | - |
? | |
| 4.2.1.99 | (2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate | the enzyme is involved in methylcitric acid cycle for propionate metabolism | Yarrowia lipolytica | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.99 | 45 | - |
- |
Yarrowia lipolytica |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.99 | 30 | - |
stable up to | Yarrowia lipolytica |
| 4.2.1.99 | 60 | - |
30 min, complete loss of activity | Yarrowia lipolytica |
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Release 2023.1 (January 2023)
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