BRENDA - Enzyme Database show

Characterization of 16alpha-hydroxyprohgesterone dehydroxylase in cell extracts of the intestinal anaerobic bacterium Eubacterium sp. 144

Glass, T.L.; Lamppa, R.S.; Hylemon, P.B.; Biochim. Biophys. Acta 792, 59-64 (1984)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.2.1.98
methanol
active at 15-30% v/v methanol with an optimal concentration of 25%
Eubacterium sp.
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.98
butanol
-
Eubacterium sp.
4.2.1.98
HgCl2
; 0.1 mM, 87% inhibition
Eubacterium sp.
4.2.1.98
iodoacetic acid
slight
Eubacterium sp.
4.2.1.98
Isopropanol
-
Eubacterium sp.
4.2.1.98
n-butanol
-
Eubacterium sp.
4.2.1.98
n-Propanol
-
Eubacterium sp.
4.2.1.98
NEM
slight
Eubacterium sp.
4.2.1.98
PCMB
0.5 mM, 50% inhibition
Eubacterium sp.
4.2.1.98
Propanol
-
Eubacterium sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.98
0.56
-
16alpha-hydroxyprogesterone
; pH 6.8, 37°C
Eubacterium sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.98
285000
-
gel filtration
Eubacterium sp.
Organic Solvent Stability
EC Number
Organic Solvent
Commentary
Organism
4.2.1.98
Methanol
high tolerance to
Eubacterium sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.98
Eubacterium sp.
-
-
-
4.2.1.98
Eubacterium sp. 144
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.98
16alpha-Hydroxypregnenolone
activity is slight less than with 16alpha-hydroxyprogesterone
5944
Eubacterium sp.
?
-
-
-
-
4.2.1.98
16alpha-Hydroxypregnenolone
activity is slight less than with 16alpha-hydroxyprogesterone
5944
Eubacterium sp. 144
?
-
-
-
-
4.2.1.98
16alpha-hydroxypregnenolone
-
5944
Eubacterium sp.
solasodin + H2O
-
-
-
?
4.2.1.98
16alpha-hydroxypregnenolone
-
5944
Eubacterium sp. 144
solasodin + H2O
-
-
-
?
4.2.1.98
16alpha-Hydroxyprogesterone
r
5944
Eubacterium sp.
16-Dehydroprogesterone
-
5944
Eubacterium sp.
-
4.2.1.98
16alpha-Hydroxyprogesterone
in the reverse reaction the enzyme is only 15% as active as in the forward reaction
5944
Eubacterium sp.
16-Dehydroprogesterone
-
5944
Eubacterium sp.
-
4.2.1.98
16alpha-Hydroxyprogesterone
r
5944
Eubacterium sp. 144
16-Dehydroprogesterone
-
5944
Eubacterium sp. 144
-
4.2.1.98
16alpha-Hydroxyprogesterone
in the reverse reaction the enzyme is only 15% as active as in the forward reaction
5944
Eubacterium sp. 144
16-Dehydroprogesterone
-
5944
Eubacterium sp. 144
-
4.2.1.98
16alpha-hydroxyprogesterone
in the hydration direction the enzyme is only 15% as active as in the dehdyration direction
5944
Eubacterium sp.
DELTA16-progesterone + H2O
-
-
-
r
4.2.1.98
DELTA16-Progesterone
-
5944
Eubacterium sp.
?
-
-
-
-
4.2.1.98
DELTA16-Progesterone
-
5944
Eubacterium sp. 144
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.98
40
45
-
Eubacterium sp.
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
4.2.1.98
35
50
35°C: about 65% of maximal activity, 50°C: about 40% of maximal activity
Eubacterium sp.
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.2.1.98
60
-
10 min, stable
Eubacterium sp.
4.2.1.98
65
-
10 min, 70% inactivation; 6 min, 70% loss of activity
Eubacterium sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.98
5.8
8
the enzyme exhibits a broad pH range without a distinct pH-optimum
Eubacterium sp.
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.2.1.98
5.8
8
broad pH-range without a distinct pH-optimum; the enzyme exhibits a broad pH range without a distinct pH-optimum. Above pH 8.5 the activity decreases and is undetectable at pH 10.0
Eubacterium sp.
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.2.1.98
methanol
active at 15-30% v/v methanol with an optimal concentration of 25%
Eubacterium sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.98
butanol
-
Eubacterium sp.
4.2.1.98
HgCl2
; 0.1 mM, 87% inhibition
Eubacterium sp.
4.2.1.98
iodoacetic acid
slight
Eubacterium sp.
4.2.1.98
Isopropanol
-
Eubacterium sp.
4.2.1.98
n-butanol
-
Eubacterium sp.
4.2.1.98
n-Propanol
-
Eubacterium sp.
4.2.1.98
NEM
slight
Eubacterium sp.
4.2.1.98
PCMB
0.5 mM, 50% inhibition
Eubacterium sp.
4.2.1.98
Propanol
-
Eubacterium sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.98
0.56
-
16alpha-hydroxyprogesterone
; pH 6.8, 37°C
Eubacterium sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.98
285000
-
gel filtration
Eubacterium sp.
Organic Solvent Stability (protein specific)
EC Number
Organic Solvent
Commentary
Organism
4.2.1.98
Methanol
high tolerance to
Eubacterium sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.98
16alpha-Hydroxypregnenolone
activity is slight less than with 16alpha-hydroxyprogesterone
5944
Eubacterium sp.
?
-
-
-
-
4.2.1.98
16alpha-Hydroxypregnenolone
activity is slight less than with 16alpha-hydroxyprogesterone
5944
Eubacterium sp. 144
?
-
-
-
-
4.2.1.98
16alpha-hydroxypregnenolone
-
5944
Eubacterium sp.
solasodin + H2O
-
-
-
?
4.2.1.98
16alpha-hydroxypregnenolone
-
5944
Eubacterium sp. 144
solasodin + H2O
-
-
-
?
4.2.1.98
16alpha-Hydroxyprogesterone
r
5944
Eubacterium sp.
16-Dehydroprogesterone
-
5944
Eubacterium sp.
-
4.2.1.98
16alpha-Hydroxyprogesterone
in the reverse reaction the enzyme is only 15% as active as in the forward reaction
5944
Eubacterium sp.
16-Dehydroprogesterone
-
5944
Eubacterium sp.
-
4.2.1.98
16alpha-Hydroxyprogesterone
r
5944
Eubacterium sp. 144
16-Dehydroprogesterone
-
5944
Eubacterium sp. 144
-
4.2.1.98
16alpha-Hydroxyprogesterone
in the reverse reaction the enzyme is only 15% as active as in the forward reaction
5944
Eubacterium sp. 144
16-Dehydroprogesterone
-
5944
Eubacterium sp. 144
-
4.2.1.98
16alpha-hydroxyprogesterone
in the hydration direction the enzyme is only 15% as active as in the dehdyration direction
5944
Eubacterium sp.
DELTA16-progesterone + H2O
-
-
-
r
4.2.1.98
DELTA16-Progesterone
-
5944
Eubacterium sp.
?
-
-
-
-
4.2.1.98
DELTA16-Progesterone
-
5944
Eubacterium sp. 144
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.2.1.98
40
45
-
Eubacterium sp.
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
4.2.1.98
35
50
35°C: about 65% of maximal activity, 50°C: about 40% of maximal activity
Eubacterium sp.
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.2.1.98
60
-
10 min, stable
Eubacterium sp.
4.2.1.98
65
-
10 min, 70% inactivation; 6 min, 70% loss of activity
Eubacterium sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.98
5.8
8
the enzyme exhibits a broad pH range without a distinct pH-optimum
Eubacterium sp.
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.2.1.98
5.8
8
broad pH-range without a distinct pH-optimum; the enzyme exhibits a broad pH range without a distinct pH-optimum. Above pH 8.5 the activity decreases and is undetectable at pH 10.0
Eubacterium sp.