EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.98 | methanol | active at 15-30% v/v methanol with an optimal concentration of 25% | Eubacterium sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.98 | butanol | - |
Eubacterium sp. | |
4.2.1.98 | HgCl2 | 0.1 mM, 87% inhibition | Eubacterium sp. | |
4.2.1.98 | iodoacetic acid | slight | Eubacterium sp. | |
4.2.1.98 | Isopropanol | - |
Eubacterium sp. | |
4.2.1.98 | n-butanol | - |
Eubacterium sp. | |
4.2.1.98 | n-Propanol | - |
Eubacterium sp. | |
4.2.1.98 | NEM | slight | Eubacterium sp. | |
4.2.1.98 | PCMB | 0.5 mM, 50% inhibition | Eubacterium sp. | |
4.2.1.98 | Propanol | - |
Eubacterium sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.98 | 0.56 | - |
16alpha-hydroxyprogesterone | - |
Eubacterium sp. | |
4.2.1.98 | 0.56 | - |
16alpha-hydroxyprogesterone | pH 6.8, 37°C | Eubacterium sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.1.98 | 285000 | - |
gel filtration | Eubacterium sp. |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
4.2.1.98 | Methanol | high tolerance to | Eubacterium sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.98 | Eubacterium sp. | - |
- |
- |
4.2.1.98 | Eubacterium sp. 144 | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.98 | 16alpha-Hydroxypregnenolone | activity is slight less than with 16alpha-hydroxyprogesterone | Eubacterium sp. | ? | - |
? | |
4.2.1.98 | 16alpha-Hydroxypregnenolone | activity is slight less than with 16alpha-hydroxyprogesterone | Eubacterium sp. 144 | ? | - |
? | |
4.2.1.98 | 16alpha-hydroxypregnenolone | - |
Eubacterium sp. | 16-dehydropregnenolone + H2O | - |
? | |
4.2.1.98 | 16alpha-hydroxypregnenolone | - |
Eubacterium sp. 144 | 16-dehydropregnenolone + H2O | - |
? | |
4.2.1.98 | 16alpha-Hydroxyprogesterone | r | Eubacterium sp. | 16-Dehydroprogesterone | - |
? | |
4.2.1.98 | 16alpha-Hydroxyprogesterone | in the reverse reaction the enzyme is only 15% as active as in the forward reaction | Eubacterium sp. | 16-Dehydroprogesterone | - |
? | |
4.2.1.98 | 16alpha-Hydroxyprogesterone | r | Eubacterium sp. 144 | 16-Dehydroprogesterone | - |
? | |
4.2.1.98 | 16alpha-Hydroxyprogesterone | in the reverse reaction the enzyme is only 15% as active as in the forward reaction | Eubacterium sp. 144 | 16-Dehydroprogesterone | - |
? | |
4.2.1.98 | 16alpha-hydroxyprogesterone | in the hydration direction the enzyme is only 15% as active as in the dehdyration direction | Eubacterium sp. | DELTA16-progesterone + H2O | - |
r | |
4.2.1.98 | DELTA16-Progesterone | - |
Eubacterium sp. | ? | - |
? | |
4.2.1.98 | DELTA16-Progesterone | - |
Eubacterium sp. 144 | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.98 | 40 | 45 | - |
Eubacterium sp. |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.98 | 35 | 50 | 35°C: about 65% of maximal activity, 50°C: about 40% of maximal activity | Eubacterium sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.98 | 60 | - |
10 min, stable | Eubacterium sp. |
4.2.1.98 | 65 | - |
6 min, 70% loss of activity | Eubacterium sp. |
4.2.1.98 | 65 | - |
10 min, 70% inactivation | Eubacterium sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.98 | 5.8 | 8 | the enzyme exhibits a broad pH range without a distinct pH-optimum | Eubacterium sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.98 | 5.8 | 8 | broad pH-range without a distinct pH-optimum | Eubacterium sp. |
4.2.1.98 | 5.8 | 8 | the enzyme exhibits a broad pH range without a distinct pH-optimum. Above pH 8.5 the activity decreases and is undetectable at pH 10.0 | Eubacterium sp. |