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Literature summary extracted from

  • Dreyer, J.L.
    The role of iron in the activation of mannonic and altronic acid hydratases, two Fe-requiring hydro-lyases (1987), Eur. J. Biochem., 166, 623-630.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.7 1,10-phenanthroline
-
Escherichia coli
4.2.1.7 alpha-picolinate
-
Escherichia coli
4.2.1.7 EDTA
-
Escherichia coli
4.2.1.7 Fe2+ low concentrations required, inhibition above 2 mM Escherichia coli
4.2.1.7 iodoacetate
-
Escherichia coli
4.2.1.7 nitrilotriacetic acid
-
Escherichia coli
4.2.1.8 1,10-phenanthroline
-
Escherichia coli
4.2.1.8 alpha-picolinate
-
Escherichia coli
4.2.1.8 EDTA
-
Escherichia coli
4.2.1.8 iodoacetate
-
Escherichia coli
4.2.1.8 nitrilotriacetic acid
-
Escherichia coli
4.2.1.8 PCMB
-
Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.7 additional information
-
additional information
-
Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.7 Fe2+ low concentrations required, inhibition above 2 mM. Upon activation the enzyme incorporates a single Fe atom. The incorporated iron is losely bound. Synergistic activation in presence of both, Mg2+ and Mn2+ Escherichia coli
4.2.1.7 Mn2+ activates, synergistic activation in presence of both, Mg2+ and Mn2+. Mn2+ appears to be a constituent of the enzyme active center Escherichia coli
4.2.1.8 Fe2+ required, upon inactivation the enzyme incorporates a single Fe atom, it contains no Fe-S core, the incorporated iron is losely bound Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.7 53000
-
x * 53000, SDS-PAGE Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.7 Escherichia coli
-
-
-
4.2.1.8 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.8
-
Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.8 203.1
-
-
Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.7 D-altronate
-
Escherichia coli 2-dehydro-3-deoxy-D-gluconate + H2O
-
?
4.2.1.8 D-Mannonate
-
Escherichia coli 2-Dehydro-3-deoxy-D-gluconate + H2O
-
?

Subunits

EC Number Subunits Comment Organism
4.2.1.7 ? x * 53000, SDS-PAGE Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.7 7.5
-
-
Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.7 6.5 8.6 about 20% of maximal activity at pH 6.5 and at pH 8.6 Escherichia coli