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Literature summary extracted from

  • Robert-Baudouy, J.; Jimeno-Abendano, J.; Stoeber, F.
    D-Mannonate and D-altronate dehydratases of Escherichia coli K12 (1982), Methods Enzymol., 90, 288-294.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.7 2-mercaptoethanol sulfhydryl compounds required, 50 mM 2-mercaptoethanol in presence of 0.8 mM FeSO4 is optimal Escherichia coli
4.2.1.8 sulfhydryl compounds required for maintenance of activity Escherichia coli

General Stability

EC Number General Stability Organism
4.2.1.7 sulfhydryl compounds stabilize Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.7 D-arabonate competitive Escherichia coli
4.2.1.7 D-glucarate weak Escherichia coli
4.2.1.8 D-glucuronate competitive Escherichia coli
4.2.1.8 D-Glucuronic amide noncompetitive Escherichia coli
4.2.1.8 D-mannitol competitive Escherichia coli
4.2.1.8 D-Mannonic amide noncompetitive Escherichia coli
4.2.1.8 D-sorbitol competitive Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.7 0.8
-
 D-altronate
-
 Escherichia coli
4.2.1.8 20
-
 D-mannonate
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.7 Fe2+ best activation at 0.8 mM Escherichia coli
4.2.1.8 Fe2+ maximal activation at 0.8 mM FeSo4 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.7 D-altronate Escherichia coli the enzyme is involved in the galacturonate branch of the hexuronate pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.7 Escherichia coli
-
-
-
4.2.1.8 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.7
-
 Escherichia coli
4.2.1.8
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.7 additional information
-
-
 Escherichia coli
4.2.1.8 additional information
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
4.2.1.7 4°C, crude extract, enzyme retains 90% of its initial activity Escherichia coli
4.2.1.8 4°C, 10 mM Tris buffer, pH 7.0, 10% loss of activity after 1 month Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.7 D-altronate
-
 Escherichia coli 2-dehydro-3-deoxy-D-gluconate + H2O
-
 ?
4.2.1.7 D-altronate the enzyme is involved in the galacturonate branch of the hexuronate pathway Escherichia coli ?
-
 ?
4.2.1.8 D-Mannonate
-
 Escherichia coli 2-Dehydro-3-deoxy-D-gluconate + H2O
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.7 59
-
 half-life: 4 min Escherichia coli
4.2.1.8 52
-
 2 min, about 50% loss of activity Escherichia coli
4.2.1.8 59
-
 1 min, 90% loss of activity Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.7 8.3
-
-
 Escherichia coli
4.2.1.8 7 8.5
-
 Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.1.8 7 8.5 maximal stability Escherichia coli