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Literature summary extracted from
- Purification et proprietes de la D-mannonate hydrolase d'Escherichia coli' (1973), Biochim. Biophys. Acta, 309, 473-485.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.8 | D-glucuronate | competitive | Escherichia coli |  |
| 4.2.1.8 | D-mannitol | competitive | Escherichia coli | |
| 4.2.1.8 | D-sorbitol | competitive | Escherichia coli | |
| 4.2.1.8 | PCMB | - |
Escherichia coli | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.8 | 20 | - |
D-mannonate | - |
Escherichia coli | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.8 | Fe2+ | FeSO4 activates | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.8 | D-mannonate | Escherichia coli | inducible by glucuronate and fructuronate | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.8 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.8 | - |
Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.8 | additional information | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.8 | D-mannonate | inducible by glucuronate and fructuronate | Escherichia coli | ? | - |
? | |
| 4.2.1.8 | D-Mannonate | - |
Escherichia coli | 2-Dehydro-3-deoxy-D-gluconate + H2O | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.8 | additional information | - |
D-gluconate protects against heat denaturation | Escherichia coli |
| 4.2.1.8 | 52 | - |
2 min, about 50% loss of activity | Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.8 | 8.3 | - |
glycylglycine buffer, in presence of FeSO4 and 2-mercaptoethanol as activator and protector | Escherichia coli |
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