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Literature summary extracted from

  • Tabuchi, T.; Aoki, H.; Uchiyama, H.; Nakahara, T.
    2-Methylcitrate dehydratase, a new enzyme functioning at the methylcitric acid cycle of propionate metabolism (1981), Agric. Biol. Chem., 45, 2823-2929.
No PubMed abstract available

General Stability

EC Number General Stability Organism
4.2.1.79 unstable in Tris-HCl buffer Yarrowia lipolytica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.79 2-Hydroxybutane-1,2,3-tricarboxylate Yarrowia lipolytica methylcitric acid cycle, propionyl-CoA oxidation ?
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 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.79 Yarrowia lipolytica
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 formerly Saccaromycopsis lipolytica
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.79 (Z)-But-2-ene-1,2,3-tricarboxylate + H2O r Yarrowia lipolytica 2-Hydroxybutane-1,2,3-tricarboxylate r ?
4.2.1.79 (Z)-But-2-ene-1,2,3-tricarboxylate + H2O r Yarrowia lipolytica 2-Hydroxybutane-1,2,3-tricarboxylate i.e. 2-methylcitrate ?
4.2.1.79 (Z)-But-2-ene-1,2,3-tricarboxylate + H2O i.e. 2-methyl-cis-aconitate Yarrowia lipolytica 2-Hydroxybutane-1,2,3-tricarboxylate r ?
4.2.1.79 (Z)-But-2-ene-1,2,3-tricarboxylate + H2O i.e. 2-methyl-cis-aconitate Yarrowia lipolytica 2-Hydroxybutane-1,2,3-tricarboxylate i.e. 2-methylcitrate ?
4.2.1.79 2-Hydroxybutane-1,2,3-tricarboxylate r Yarrowia lipolytica (Z)-But-2-ene-1,2,3-tricarboxylate + H2O r ?
4.2.1.79 2-Hydroxybutane-1,2,3-tricarboxylate i.e. 2-methylcitrate Yarrowia lipolytica (Z)-But-2-ene-1,2,3-tricarboxylate + H2O r ?
4.2.1.79 2-Hydroxybutane-1,2,3-tricarboxylate methylcitric acid cycle, propionyl-CoA oxidation Yarrowia lipolytica ?
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 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.79 25 40
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 Yarrowia lipolytica

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.1.79 15 45
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 Yarrowia lipolytica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.79 40
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 30 min, stable Yarrowia lipolytica
4.2.1.79 60
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 30 min, complete inactivation Yarrowia lipolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.79 6.5 7
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 Yarrowia lipolytica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.79 5.5 8.5
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 Yarrowia lipolytica

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.1.79 6.5 7.5
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 Yarrowia lipolytica