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Literature summary extracted from
- Purification and properties of uroporphyrinogen III synthase (co-synthase) from an overproducing recombinant strain of Escherichia coli K-12 (1989), Biochem. J., 264, 397-402.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.75 | overproduction | Escherichia coli |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 4.2.1.75 | purification needs to be carried out as close to 0¦C as possible | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.75 | 5,5'-dithiobis(2-nitrobenzoate) | - |
Escherichia coli |  |
| 4.2.1.75 | diethyl dicarbonate | - |
Escherichia coli | |
| 4.2.1.75 | iodoacetamide | - |
Escherichia coli | |
| 4.2.1.75 | iodoacetic acid | - |
Escherichia coli | |
| 4.2.1.75 | N-ethylmaleimide | - |
Escherichia coli | |
| 4.2.1.75 | p-chloromercuribenzoate | - |
Escherichia coli | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.75 | 0.005 | - |
Hydroxymethylbilane | - |
Escherichia coli | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.75 | additional information | bivalent ions fail to activate or stabilize the enzyme significantly | Escherichia coli |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.75 | 26000 | - |
SDS-PAGE | Escherichia coli |
| 4.2.1.75 | 29000 | - |
gel filtration | Escherichia coli |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.75 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.75 | from overproducing strain | Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.75 | 25 | - |
hydroxymethylbilane | Escherichia coli |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.2.1.75 | -20¦C, stable to freezing | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.75 | Hydroxymethylbilane | - |
Escherichia coli | Uroporphyrinogen III | - |
? | |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.75 | 20 | - |
20% loss of activity in 10 min | Escherichia coli |
| 4.2.1.75 | 60 | - |
completely inactivated in 1 min | Escherichia coli |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.75 | 500 | - |
Hydroxymethylbilane | - |
Escherichia coli | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.75 | 7.8 | - |
- |
Escherichia coli |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.75 | 7 | 9 | at pH 7.0: 60% of optimum activity, at pH 9.0: 60% of optimum activity | Escherichia coli |
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