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Literature summary extracted from
- Cyanide metabolism in higher plants. V. The formation of asparagine from beta-cyanoalanine (1972), Arch. Biochem. Biophys., 152, 62-69.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.65 | AgNO3 | - |
Lupinus angustifolius |  |
| 4.2.1.65 | HgCl2 | - |
Lupinus angustifolius | |
| 4.2.1.65 | iodoacetic acid | - |
Lupinus angustifolius | |
| 4.2.1.65 | N-ethylmaleimide | - |
Lupinus angustifolius | |
| 4.2.1.65 | NaCN | - |
Lupinus angustifolius | |
| 4.2.1.65 | Zn(CH3COO)2 | - |
Lupinus angustifolius | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.65 | 2 | - |
Beta-cyano-L-alanine | - |
Lupinus angustifolius | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.2.1.65 | soluble | - |
Lupinus angustifolius | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.65 | 400000 | 500000 | gel filtration | Lupinus angustifolius |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.65 | Lupinus angustifolius | - |
- |
- |
| 4.2.1.65 | Sorghum bicolor | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.65 | - |
Lupinus angustifolius |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.2.1.65 | cotyledon | - |
Lupinus angustifolius | - |
| 4.2.1.65 | root | - |
Lupinus angustifolius | - |
| 4.2.1.65 | seedling | - |
Lupinus angustifolius | - |
| 4.2.1.65 | shoot | - |
Lupinus angustifolius | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.65 | 0.371 | - |
- |
Sorghum bicolor |
| 4.2.1.65 | 0.371 | - |
- |
Lupinus angustifolius |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.65 | beta-Cyano-L-alanine + H2O | - |
Sorghum bicolor | L-Asparagine | - |
? | |
| 4.2.1.65 | beta-Cyano-L-alanine + H2O | - |
Lupinus angustifolius | L-Asparagine | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.65 | 8 | - |
- |
Lupinus angustifolius |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.65 | 6.5 | 9 | - |
Lupinus angustifolius |
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Release 2023.1 (January 2023)
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