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Literature summary extracted from

  • Martinez-Blanco, H.; Reglero, A.; Fenandez-Valverde, M.; Ferrero, M.A.; Moreno, M.A.; Penalva, M.A.; Luengo, J.M.
    Isolation and characterization of the acetyl-CoA synthetase from Penicillium chrysogenum (1992), J. Biol. Chem., 267, 5474-5481.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.2.1.1 2-mercaptoethanol stimulates Penicillium chrysogenum
6.2.1.1 DTT stimulates Penicillium chrysogenum
6.2.1.1 GSH stimulates Penicillium chrysogenum

General Stability

EC Number General Stability Organism
6.2.1.1 particularly sensitive to repeated freezing Penicillium chrysogenum

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.1 5,5'-dithiobis(2-nitrobenzoate)
-
 Penicillium chrysogenum
6.2.1.1 Hg2+
-
 Penicillium chrysogenum
6.2.1.1 NEM
-
 Penicillium chrysogenum
6.2.1.1 Nonidet P40 weak Penicillium chrysogenum
6.2.1.1 p-chloromercuribenzoate
-
 Penicillium chrysogenum
6.2.1.1 Tween 100
-
 Penicillium chrysogenum
6.2.1.1 Tween 20
-
 Penicillium chrysogenum
6.2.1.1 Tween 80
-
 Penicillium chrysogenum
6.2.1.1 Zn2+
-
 Penicillium chrysogenum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.1 0.18
-
 CoA
-
 Penicillium chrysogenum
6.2.1.1 6.8
-
 acetate
-
 Penicillium chrysogenum
6.2.1.1 17
-
 ATP
-
 Penicillium chrysogenum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.1 Mg2+ required Penicillium chrysogenum
6.2.1.1 Mn2+ can replace Mg2+ in activation Penicillium chrysogenum
6.2.1.1 Mn2+ 64% of the activation relative to Mg2+ Penicillium chrysogenum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.1 70000
-
 2 * 70000, SDS-PAGE Penicillium chrysogenum
6.2.1.1 139000
-
 gel filtration Penicillium chrysogenum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.1 additional information Aspergillus nidulans the enzyme can catalyze the activation to their CoA thioesters of some of the side-chain precursors required in Penicillium chrysogenum and Aspergillus nidulans for the production of several penicillins ?
-
 ?
6.2.1.1 additional information Penicillium chrysogenum the enzyme can catalyze the activation to their CoA thioesters of some of the side-chain precursors required in Penicillium chrysogenum and Aspergillus nidulans for the production of several penicillins ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.1 Aspergillus nidulans
-
-
-
6.2.1.1 Penicillium chrysogenum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.1
-
 Penicillium chrysogenum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.1 mycelium
-
 Penicillium chrysogenum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.1 24
-
-
 Penicillium chrysogenum

Storage Stability

EC Number Storage Stability Organism
6.2.1.1 -20°C, cannot be stored for more than 3 days without a considerable loss of activity Penicillium chrysogenum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.1 ADP + acetate + CoA 37% of the activity relative to ATP Penicillium chrysogenum AMP + diphosphate + acetyl-CoA
-
 ?
6.2.1.1 ATP + acetate + CoA
-
 Aspergillus nidulans AMP + diphosphate + acetyl-CoA
-
 ?
6.2.1.1 ATP + acetate + CoA
-
 Penicillium chrysogenum AMP + diphosphate + acetyl-CoA
-
 ?
6.2.1.1 ATP + butyrate + CoA 20% of the activity relative to acetate Penicillium chrysogenum AMP + diphosphate + butyryl-CoA
-
 ?
6.2.1.1 ATP + pentanoate + CoA 6.7% of the activity relative to acetate Penicillium chrysogenum AMP + diphosphate + pentanoyl-CoA
-
 ?
6.2.1.1 ATP + propanoate + CoA 48% of the activity relative to acetate Penicillium chrysogenum AMP + diphosphate + propanoyl-CoA
-
 ?
6.2.1.1 CTP + acetate + CoA
-
 Penicillium chrysogenum CMP + diphosphate + acetyl-CoA
-
 ?
6.2.1.1 GTP + acetate + CoA
-
 Penicillium chrysogenum GMP + diphosphate + acetyl-CoA
-
 ?
6.2.1.1 additional information the enzyme can activate many other molecules to acyl-CoA derivatives: hexanoate, 3-hexenoate, heptanoate, octanoate, 3-octenoate, phenylacetate, 2-thiophene acetate, 3-thiophene acetate Penicillium chrysogenum ?
-
 ?
6.2.1.1 additional information the enzyme can catalyze the activation to their CoA thioesters of some of the side-chain precursors required in Penicillium chrysogenum and Aspergillus nidulans for the production of several penicillins Aspergillus nidulans ?
-
 ?
6.2.1.1 additional information the enzyme can catalyze the activation to their CoA thioesters of some of the side-chain precursors required in Penicillium chrysogenum and Aspergillus nidulans for the production of several penicillins Penicillium chrysogenum ?
-
 ?
6.2.1.1 UTP + acetate + CoA
-
 Penicillium chrysogenum UMP + diphosphate + acetyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.1 dimer 2 * 70000, SDS-PAGE Penicillium chrysogenum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.1 37
-
-
 Penicillium chrysogenum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.2.1.1 20 50 20°C: about 45% of maximal activity, 50°C: about 30% of maximal activity Penicillium chrysogenum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.1 8
-
-
 Penicillium chrysogenum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.2.1.1 6 9.7 6.0: about 45% of maximal activity, 9.7: about 70% of maximal activity Penicillium chrysogenum