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Literature summary extracted from

  • Romana, L.K.; Santiago, F.S.; Reeves, P.R.
    High level expression and purification of dThymidine diphospho-D-glucose 4,6-dehydratase (rfbB) from Salmonella serovar typhimurium LT2 (1991), Biochem. Biophys. Res. Commun., 174, 846-852.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.46 expression in Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.46 p-chloromercuriphenylsulfonate complete inactivation with 0.01M; complete reactivation after addition of 2-mercaptoethanol, NAD+ and cysteine Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.46 0.427
-
 dTDPglucose
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.46 43000
-
 2 * 43000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.46 77000
-
 gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.46 dTDP-glucose Salmonella enterica subsp. enterica serovar Typhimurium
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.46 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.46 expression in Escherichia coli Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.46 930
-
 dTDPglucose Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.46 dTDP-glucose
-
 Salmonella enterica subsp. enterica serovar Typhimurium dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
 ?
4.2.1.46 dTDP-glucose
-
 Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
4.2.1.46 additional information not: ADPglucose, CDPglucose, UDPglucose, GDPglucose, UDPgalactose, dTDPmannose, CTP and dTTP Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.46 dimer 2 * 43000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.46 35
-
 dTDPglucose Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.46 8.5
-
 dTDPglucose Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.46 NAD+
-
 Salmonella enterica subsp. enterica serovar Typhimurium