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Literature summary extracted from

  • Hopper, D.J.; Kemp, P.D.
    Regulation of enzymes of the 3,5-xylenol-degradative pathway in Pseudomonas putida: evidence for a plasmid (1980), J. Bacteriol., 142, 21-26.
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.35 2-Methylmaleate + H2O Pseudomonas putida enzyme in the pathway of metabolism of 3,5-xylenol and oxidation of p-cresol ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.97 Pseudomonas putida
-
-
-
4.2.1.35 Pseudomonas putida
-
NCIB 9869
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.97 0.57
-
enzyme activity in crude extracts from cells grown on 3,5-xylenol Pseudomonas putida
1.1.1.97 0.65
-
enzyme activity in crude extracts from cells grown on 3-hydroxy-5-methylbenzoate Pseudomonas putida
1.1.1.97 0.7
-
enzyme activity in crude extracts from cells grown on gentisate Pseudomonas putida
1.1.1.97 0.78
-
enzyme activity in crude extracts from cells grown on m-hxdroxybenzoate Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.97 3-hydroxybenzyl alcohol + NADP+
-
Pseudomonas putida 3-hydroxybenzaldehyde + NADPH
-
?
4.2.1.35 2-Methylmaleate + H2O
-
Pseudomonas putida (R)-2-Methylmalate
-
?
4.2.1.35 2-Methylmaleate + H2O enzyme in the pathway of metabolism of 3,5-xylenol and oxidation of p-cresol Pseudomonas putida ?
-
?