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Literature summary extracted from

  • Subramanian, S.S.; Raghavendra Rao, M.R.
    Purification and properties of citraconase (1968), J. Biol. Chem., 243, 2367-2372.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.35 Cys sulfhydryl compound is required for maximal activity Pseudomonas sp.
4.2.1.35 GSH sulfhydryl compound is required for maximal activity Pseudomonas sp.
4.2.1.35 mercaptoethanol sulfhydryl compound is required for maximal activity Pseudomonas sp.
4.2.1.35 Thioglycollate sulfhydryl compound is required for maximal activity Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.35 2,2'-dipyridyl 1 mM, 99% inhibition in absence of GSH, 74% inhibition in presence of 0.5 mM GSH Pseudomonas sp.
4.2.1.35 Cu2+
-
Pseudomonas sp.
4.2.1.35 diphosphate 1 mM, 99% inhibition in absence of GSH, 74% inhibition in presence of 0.5 mM GSH Pseudomonas sp.
4.2.1.35 EDTA 1 mM, 99% inhibition in absence of GSH, 74% inhibition in presence of 0.5 mM GSH Pseudomonas sp.
4.2.1.35 Hg2+
-
Pseudomonas sp.
4.2.1.35 iodoacetate
-
Pseudomonas sp.
4.2.1.35 PCMB
-
Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.35 1.1
-
2-methylmaleate
-
Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.35 Fe2+ required for maximal activity Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.35 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.35
-
Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.35 additional information
-
-
Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.35 2-Methylmaleate + H2O
-
Pseudomonas sp. (R)-2-Methylmalate i.e. L-citramalate ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.35 7.5
-
-
Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.35 6.3 8.2 pH 6.3: about 30% of maximal activity, pH 8.2: about 55% of maximal activity Pseudomonas sp.