EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.35 | Cys | sulfhydryl compound is required for maximal activity | Pseudomonas sp. | |
4.2.1.35 | GSH | sulfhydryl compound is required for maximal activity | Pseudomonas sp. | |
4.2.1.35 | mercaptoethanol | sulfhydryl compound is required for maximal activity | Pseudomonas sp. | |
4.2.1.35 | Thioglycollate | sulfhydryl compound is required for maximal activity | Pseudomonas sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.35 | 2,2'-dipyridyl | 1 mM, 99% inhibition in absence of GSH, 74% inhibition in presence of 0.5 mM GSH | Pseudomonas sp. | |
4.2.1.35 | Cu2+ | - |
Pseudomonas sp. | |
4.2.1.35 | diphosphate | 1 mM, 99% inhibition in absence of GSH, 74% inhibition in presence of 0.5 mM GSH | Pseudomonas sp. | |
4.2.1.35 | EDTA | 1 mM, 99% inhibition in absence of GSH, 74% inhibition in presence of 0.5 mM GSH | Pseudomonas sp. | |
4.2.1.35 | Hg2+ | - |
Pseudomonas sp. | |
4.2.1.35 | iodoacetate | - |
Pseudomonas sp. | |
4.2.1.35 | PCMB | - |
Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.35 | 1.1 | - |
2-methylmaleate | - |
Pseudomonas sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.35 | Fe2+ | required for maximal activity | Pseudomonas sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.35 | Pseudomonas sp. | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.35 | - |
Pseudomonas sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.1.35 | additional information | - |
- |
Pseudomonas sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.35 | 2-Methylmaleate + H2O | - |
Pseudomonas sp. | (R)-2-Methylmalate | i.e. L-citramalate | ? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.35 | 7.5 | - |
- |
Pseudomonas sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.35 | 6.3 | 8.2 | pH 6.3: about 30% of maximal activity, pH 8.2: about 55% of maximal activity | Pseudomonas sp. |