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Literature summary extracted from

  • Kato, Y.; Asano, Y.
    3-Methylaspartate ammonia-lyase as a marker enzyme of the mesaconate pathway for (S)-glutamate fermentation in Enterobacteriaceae (1997), Arch. Microbiol., 168, 457-463.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.3.1.2 Dimethylsulfoxide enhances activity Citrobacter freundii
4.3.1.2 Dimethylsulfoxide enhances activity Morganella morganii

Application

EC Number Application Comment Organism
4.3.1.2 analysis a marker enzyme of the mesaconate pathway for (S)-glutamate fermentation in Enterobacteriaceae Citrobacter freundii
4.3.1.2 analysis a marker enzyme of the mesaconate pathway for (S)-glutamate fermentation in Enterobacteriaceae Morganella morganii
4.3.1.2 analysis a marker enzyme of the mesaconate pathway for (S)-glutamate fermentation in Enterobacteriaceae Raoultella planticola
4.3.1.2 analysis a marker enzyme of the mesaconate pathway for (S)-glutamate fermentation in Enterobacteriaceae Citrobacter koseri
4.3.1.2 analysis a marker enzyme of the mesaconate pathway for (S)-glutamate fermentation in Enterobacteriaceae Citrobacter amalonaticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.2 40000
-
2 * 40000, SDS-PAGE Citrobacter freundii
4.3.1.2 40000
-
2 * 40000, SDS-PAGE Citrobacter koseri
4.3.1.2 42000
-
2 * 42000, SDS-PAGE Citrobacter amalonaticus
4.3.1.2 44000
-
2 * 44000, SDS-PAGE Morganella morganii
4.3.1.2 45000
-
2 * 45000, SDS-PAGE Raoultella planticola

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.34 additional information Clostridium sp. key enzyme of the mesaconate pathway of (S)-glutamate fermentation ?
-
?
4.3.1.2 L-threo-3-methylaspartate Citrobacter freundii
-
?
-
?
4.3.1.2 L-threo-3-methylaspartate Clostridium tetanomorphum
-
?
-
?
4.3.1.2 L-threo-3-methylaspartate Morganella morganii
-
?
-
?
4.3.1.2 L-threo-3-methylaspartate Raoultella planticola
-
?
-
?
4.3.1.2 L-threo-3-methylaspartate Citrobacter koseri
-
?
-
?
4.3.1.2 L-threo-3-methylaspartate Citrobacter amalonaticus
-
?
-
?
4.3.1.2 L-threo-3-methylaspartate Citrobacter amalonaticus YG-1002
-
?
-
?
4.3.1.2 L-threo-3-methylaspartate Citrobacter amalonaticus YG1002
-
?
-
?
4.3.1.2 L-threo-3-methylaspartate Citrobacter freundii YG-0504
-
?
-
?
4.3.1.2 L-threo-3-methylaspartate Morganella morganii NCIMB 232
-
?
-
?
4.3.1.2 L-threo-3-methylaspartate Raoultella planticola IAM 1133
-
?
-
?
4.3.1.2 L-threo-3-methylaspartate Citrobacter freundii TPU 0011
-
?
-
?
4.3.1.2 L-threo-3-methylaspartate Citrobacter koseri JCM 1659
-
?
-
?
4.3.1.2 L-threo-3-methylaspartate Morganella morganii NCIMB 9525
-
?
-
?
4.3.1.2 L-threo-3-methylaspartate Citrobacter koseri JCM 1658
-
?
-
?
4.3.1.2 L-threo-3-methylaspartate Morganella morganii YG-0601
-
?
-
?
4.3.1.2 L-threo-3-methylaspartate Morganella morganii NCIMB 10466
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.34 Citrobacter amalonaticus
-
YG1002
-
4.2.1.34 Citrobacter amalonaticus YG1002
-
YG1002
-
4.2.1.34 Citrobacter freundii
-
YG-0504
-
4.2.1.34 Citrobacter freundii YG-0504
-
YG-0504
-
4.2.1.34 Clostridium sp.
-
-
-
4.2.1.34 Morganella morganii
-
YG-0601
-
4.2.1.34 Morganella morganii YG-0601
-
YG-0601
-
4.2.1.34 Raoultella planticola
-
IAM 1133
-
4.2.1.34 Raoultella planticola IAM 1133
-
IAM 1133
-
4.3.1.2 Citrobacter amalonaticus
-
formerly Escherichia coli
-
4.3.1.2 Citrobacter amalonaticus YG-1002
-
formerly Escherichia coli
-
4.3.1.2 Citrobacter freundii
-
-
-
4.3.1.2 Citrobacter freundii
-
IFO 13545
-
4.3.1.2 Citrobacter freundii
-
IFO 13539
-
4.3.1.2 Citrobacter freundii
-
TPU 0011
-
4.3.1.2 Citrobacter freundii
-
IFO 13546
-
4.3.1.2 Citrobacter freundii TPU 0011
-
TPU 0011
-
4.3.1.2 Citrobacter freundii YG-0504
-
-
-
4.3.1.2 Citrobacter koseri
-
JCM 1659
-
4.3.1.2 Citrobacter koseri
-
JCM 1658
-
4.3.1.2 Citrobacter koseri JCM 1658
-
JCM 1658
-
4.3.1.2 Citrobacter koseri JCM 1659
-
JCM 1659
-
4.3.1.2 Clostridium tetanomorphum
-
-
-
4.3.1.2 Morganella morganii
-
-
-
4.3.1.2 Morganella morganii
-
NCTC 1707
-
4.3.1.2 Morganella morganii
-
NCTC 2815
-
4.3.1.2 Morganella morganii
-
NCIMB 232
-
4.3.1.2 Morganella morganii
-
NCTC 10011
-
4.3.1.2 Morganella morganii
-
NCIMB 9525
-
4.3.1.2 Morganella morganii
-
NCTC 5845
-
4.3.1.2 Morganella morganii
-
NCIMB 10466
-
4.3.1.2 Morganella morganii
-
IFO 3168
-
4.3.1.2 Morganella morganii
-
NCTC 2818
-
4.3.1.2 Morganella morganii
-
NCTC 11354
-
4.3.1.2 Morganella morganii
-
NCTC 12028
-
4.3.1.2 Morganella morganii
-
IFO 3848
-
4.3.1.2 Morganella morganii
-
NCTC 10375
-
4.3.1.2 Morganella morganii
-
NCTC 7381
-
4.3.1.2 Morganella morganii NCIMB 10466
-
NCIMB 10466
-
4.3.1.2 Morganella morganii NCIMB 232
-
NCIMB 232
-
4.3.1.2 Morganella morganii NCIMB 9525
-
NCIMB 9525
-
4.3.1.2 Morganella morganii YG-0601
-
-
-
4.3.1.2 Raoultella planticola
-
IAM 1133
-
4.3.1.2 Raoultella planticola IAM 1133
-
IAM 1133
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.3.1.2 enzyme production is suppressed by an incorporation of aerobic cultivation before growth under oxygen-limited conditions Citrobacter freundii
4.3.1.2 enzyme production is suppressed by an incorporation of aerobic cultivation before growth under oxygen-limited conditions Morganella morganii
4.3.1.2 enzyme production is suppressed by an incorporation of aerobic cultivation before growth under oxygen-limited conditions Raoultella planticola
4.3.1.2 additional information Citrobacter freundii
4.3.1.2 additional information Morganella morganii
4.3.1.2 additional information Raoultella planticola
4.3.1.2 additional information Citrobacter amalonaticus

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.2 IAM 1133 Raoultella planticola
4.3.1.2 JCM 1658, JCM 1659 Citrobacter koseri
4.3.1.2 YG-0504, TPU 0011, IFO 13539, IFO 13545, IFO 13546 Citrobacter freundii
4.3.1.2 YG-0601, IFO 3168, IFO 3848, NCIMB 232, NCIMB 9525, NCIMB 10466, NCTC 1707, NCTC 2815, NCTC 2818, NCTC 5845, NCTC 7381, NCTC10011, NCTC 10375, NCTC11354, NCTC 12028 Morganella morganii
4.3.1.2 YG-1002, IFO 13547 Citrobacter amalonaticus

Reaction

EC Number Reaction Comment Organism Reaction ID
4.3.1.2 L-threo-3-methylaspartate = mesaconate + NH3 mechanism Citrobacter freundii
4.3.1.2 L-threo-3-methylaspartate = mesaconate + NH3 mechanism Clostridium tetanomorphum
4.3.1.2 L-threo-3-methylaspartate = mesaconate + NH3 mechanism Morganella morganii
4.3.1.2 L-threo-3-methylaspartate = mesaconate + NH3 mechanism Raoultella planticola
4.3.1.2 L-threo-3-methylaspartate = mesaconate + NH3 mechanism Citrobacter koseri
4.3.1.2 L-threo-3-methylaspartate = mesaconate + NH3 mechanism Citrobacter amalonaticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.2 additional information
-
-
Citrobacter freundii
4.3.1.2 additional information
-
-
Clostridium tetanomorphum
4.3.1.2 additional information
-
-
Morganella morganii
4.3.1.2 additional information
-
-
Raoultella planticola
4.3.1.2 additional information
-
-
Citrobacter koseri
4.3.1.2 additional information
-
-
Citrobacter amalonaticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.34 Citramalate + H2O
-
Clostridium sp. Mesaconate
-
?
4.2.1.34 Citramalate + H2O
-
Morganella morganii Mesaconate
-
?
4.2.1.34 Citramalate + H2O
-
Raoultella planticola Mesaconate
-
?
4.2.1.34 Citramalate + H2O
-
Citrobacter amalonaticus Mesaconate
-
?
4.2.1.34 Citramalate + H2O
-
Citrobacter amalonaticus YG-1002 Mesaconate
-
?
4.2.1.34 Citramalate + H2O
-
Citrobacter amalonaticus YG1002 Mesaconate
-
?
4.2.1.34 Citramalate + H2O
-
Morganella morganii NCIMB 232 Mesaconate
-
?
4.2.1.34 Citramalate + H2O
-
Raoultella planticola IAM 1133 Mesaconate
-
?
4.2.1.34 Citramalate + H2O
-
Morganella morganii NCIMB 9525 Mesaconate
-
?
4.2.1.34 Citramalate + H2O
-
Morganella morganii YG-0601 Mesaconate
-
?
4.2.1.34 Citramalate + H2O
-
Morganella morganii NCIMB 10466 Mesaconate
-
?
4.2.1.34 additional information key enzyme of the mesaconate pathway of (S)-glutamate fermentation Clostridium sp. ?
-
?
4.3.1.2 L-threo-3-methylaspartate r Citrobacter freundii mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate r Clostridium tetanomorphum mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate r Morganella morganii mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate r Raoultella planticola mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate r Citrobacter koseri mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate r Citrobacter amalonaticus mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Citrobacter freundii mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Clostridium tetanomorphum mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Morganella morganii mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Raoultella planticola mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Citrobacter koseri mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Citrobacter amalonaticus mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate r Citrobacter amalonaticus YG-1002 mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Citrobacter amalonaticus YG-1002 mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate r Citrobacter amalonaticus YG1002 mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Citrobacter amalonaticus YG1002 mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate r Citrobacter freundii YG-0504 mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Citrobacter freundii YG-0504 mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate r Morganella morganii NCIMB 232 mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Morganella morganii NCIMB 232 mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate r Raoultella planticola IAM 1133 mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Raoultella planticola IAM 1133 mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate r Citrobacter freundii TPU 0011 mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Citrobacter freundii TPU 0011 mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate r Citrobacter koseri JCM 1659 mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Citrobacter koseri JCM 1659 mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate r Morganella morganii NCIMB 9525 mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Morganella morganii NCIMB 9525 mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate r Citrobacter koseri JCM 1658 mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Citrobacter koseri JCM 1658 mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate r Morganella morganii YG-0601 mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Morganella morganii YG-0601 mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate r Morganella morganii NCIMB 10466 mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate (2S,3S)-3-methylaspartic acid Morganella morganii NCIMB 10466 mesaconate + NH3
-
?
4.3.1.2 L-threo-3-methylaspartate
-
Citrobacter freundii ?
-
?
4.3.1.2 L-threo-3-methylaspartate
-
Clostridium tetanomorphum ?
-
?
4.3.1.2 L-threo-3-methylaspartate
-
Morganella morganii ?
-
?
4.3.1.2 L-threo-3-methylaspartate
-
Raoultella planticola ?
-
?
4.3.1.2 L-threo-3-methylaspartate
-
Citrobacter koseri ?
-
?
4.3.1.2 L-threo-3-methylaspartate
-
Citrobacter amalonaticus ?
-
?
4.3.1.2 L-threo-3-methylaspartate
-
Citrobacter amalonaticus YG-1002 ?
-
?
4.3.1.2 L-threo-3-methylaspartate
-
Citrobacter amalonaticus YG1002 ?
-
?
4.3.1.2 L-threo-3-methylaspartate
-
Citrobacter freundii YG-0504 ?
-
?
4.3.1.2 L-threo-3-methylaspartate
-
Morganella morganii NCIMB 232 ?
-
?
4.3.1.2 L-threo-3-methylaspartate
-
Raoultella planticola IAM 1133 ?
-
?
4.3.1.2 L-threo-3-methylaspartate
-
Citrobacter freundii TPU 0011 ?
-
?
4.3.1.2 L-threo-3-methylaspartate
-
Citrobacter koseri JCM 1659 ?
-
?
4.3.1.2 L-threo-3-methylaspartate
-
Morganella morganii NCIMB 9525 ?
-
?
4.3.1.2 L-threo-3-methylaspartate
-
Citrobacter koseri JCM 1658 ?
-
?
4.3.1.2 L-threo-3-methylaspartate
-
Morganella morganii YG-0601 ?
-
?
4.3.1.2 L-threo-3-methylaspartate
-
Morganella morganii NCIMB 10466 ?
-
?

Subunits

EC Number Subunits Comment Organism
4.3.1.2 dimer
-
Clostridium tetanomorphum
4.3.1.2 dimer 2 * 45000, SDS-PAGE Raoultella planticola
4.3.1.2 dimer 2 * 44000, SDS-PAGE Morganella morganii
4.3.1.2 dimer 2 * 40000, SDS-PAGE Citrobacter freundii
4.3.1.2 dimer 2 * 40000, SDS-PAGE Citrobacter koseri
4.3.1.2 dimer 2 * 42000, SDS-PAGE Citrobacter amalonaticus