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Literature summary extracted from

  • Suzuki, S.; Osumi, T.; Katsuki, H.
    Properties and metabolic role of mesaconate hydratase of an aerobic bacterium (1977), J. Biochem., 81, 1917-1925.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.34 PCMB 0.15 mM, complete inhibition. 80% protection by 5 mM S-citramalate Aerobic bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.34 0.07
-
mesaconate
-
Aerobic bacterium
4.2.1.34 0.11
-
fumarate
-
Aerobic bacterium
4.2.1.34 0.45
-
S-citramalate
-
Aerobic bacterium
4.2.1.34 0.56
-
S-malate
-
Aerobic bacterium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.34 160000
-
gel filtration Aerobic bacterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.34 additional information Aerobic bacterium constitutive enzyme, the enzyme may play a role in the interconversion between S-malate and fumarate in addition to that between S-citramalate and mesaconate ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.34 Aerobic bacterium
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.2.1.34 4°C, 90% loss of activity after 24 h in air. Reactivation by Fe2+ and 2-mercaptoethanol Aerobic bacterium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.34 7.1
-
-
Aerobic bacterium

Storage Stability

EC Number Storage Stability Organism
4.2.1.34 -20°C, in presence of 20% (NH4)2SO4 or 10% glycerol in air, about 80-90% can be reactivated even after several months Aerobic bacterium
4.2.1.34 4°C, 70% loss of activity after 1 week under N2 Aerobic bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.34 (L)-citramalate
-
Aerobic bacterium mesaconate + H2O
-
?
4.2.1.34 (S)-malate
-
Aerobic bacterium fumarate + H2O
-
?
4.2.1.34 Citramalate + H2O
-
Aerobic bacterium Mesaconate
-
?
4.2.1.34 fumarate + H2O
-
Aerobic bacterium L-malate
-
?
4.2.1.34 mesaconate
-
Aerobic bacterium citramalate + H2O
-
?
4.2.1.34 additional information constitutive enzyme, the enzyme may play a role in the interconversion between S-malate and fumarate in addition to that between S-citramalate and mesaconate Aerobic bacterium ?
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.34 8.5
-
with mesaconate as substrate Aerobic bacterium
4.2.1.34 8.7
-
with fumarate as substrate Aerobic bacterium
4.2.1.34 9
-
S-malate as substrate Aerobic bacterium
4.2.1.34 9.1
-
with S-citramalate as substrate Aerobic bacterium