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Literature summary extracted from

  • Gross, S.R.; Umbarger, H.E.
    The biosynthesis of leucine: II. The enzymic isomerization of beta-carboxy-beta-hydroxyisocaproate and alpha-hydroxy-beta-carboxyisocaproate (1963), Biochemistry, 2, 1046-1052.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.33 additional information no requirement Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.33 additional information no requirement Neurospora crassa

General Stability

EC Number General Stability Organism
4.2.1.33 10% inactivation at 0°C in phosphate buffer at pH 7 or above Neurospora crassa
4.2.1.33 loss of activity after repeated freezing and thawing Neurospora crassa

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.33 beta-carboxy-beta-hydroxyisocaproate synthetic, racemic mixture Neurospora crassa
4.2.1.33 beta-carboxy-beta-hydroxyisocaproate synthetic, racemic mixture Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.33 cysteine 55% inhibition at 20 mM, protected by substrate Neurospora crassa
4.2.1.33 cysteine
-
 Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.33 glutathione protection by substrate Neurospora crassa
4.2.1.33 glutathione protection by substrate Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.33 mercaptoethanol protection by substrate Neurospora crassa
4.2.1.33 mercaptoethanol protection by substrate Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.33 additional information additional compounds tested, without effect Neurospora crassa
4.2.1.33 additional information additional compounds tested, without effect Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.33 N-ethylmaleimide
-
 Neurospora crassa
4.2.1.33 N-ethylmaleimide
-
 Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.33 sulfhydryl compounds protection by substrate Neurospora crassa
4.2.1.33 sulfhydryl compounds protection by substrate Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.33 0.078
-
 alpha-hydroxy-beta-carboxyisocaproate
-
 Neurospora crassa
4.2.1.33 0.18
-
 dimethylcitraconate
-
 Neurospora crassa
4.2.1.33 0.95
-
 beta-carboxy-beta-hydroxyisocaproate
-
 Neurospora crassa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.33 additional information no requirement Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.33 additional information no requirement Neurospora crassa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.33 70000 90000 sucrose density gradient centrifugation Neurospora crassa

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.33 Neurospora crassa
-
-
-
4.2.1.33 Salmonella enterica subsp. enterica serovar Typhimurium
-
 wild type and Leu- -mutants
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.33 (2R,3S)-3-isopropylmalate = 2-isopropylmaleate + H2O equilibrium concentration ratios Neurospora crassa
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.33 6.2
-
-
 Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.33 2-isopropylmaleate + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium 3-isopropylmalate
-
 ?
4.2.1.33 2-isopropylmaleate + H2O
-
 Neurospora crassa 3-isopropylmalate
-
 ?
4.2.1.33 3-isopropylmalate
-
 Salmonella enterica subsp. enterica serovar Typhimurium 2-isopropylmaleate + H2O
-
 ?
4.2.1.33 3-isopropylmalate
-
 Neurospora crassa 2-isopropylmaleate + H2O
-
 ?
4.2.1.33 beta-carboxy-beta-hydroxyisocaproate
-
 Neurospora crassa ?
-
 ?
4.2.1.33 dimethylcitraconate
-
 Neurospora crassa ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.33 32
-
 enzyme assay at Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.33 32
-
 enzyme assay at Neurospora crassa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.33 45
-
 50% loss of activity after 2 min Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.33 7
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.33 7
-
-
 Neurospora crassa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.33 6 8 at pH 6.0, 20% of optimum activity, activity varies only about 20% between pH 7.0 and 8.0 Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.33 6 8 at pH 6.0, 20% of optimum activity, activity varies only about 20% between pH 7.0 and 8.0 Neurospora crassa