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Literature summary extracted from

  • Kelly, J.M.; Scopes, R.K.
    L-(+)-Tartrate dehydratase from Pseudomonas putida is an iron-sulphur enzyme (1986), FEBS Lett., 202, 274-276.
No PubMed abstract available

General Stability

EC Number General Stability Organism
4.2.1.32 manipulations on dye-ligand columns cause rapid irrecoverable loss of activity Pseudomonas putida
4.2.1.32 sucrose and/or succinate stabilize Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.32 Tartronic acid
-
 Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.32 Fe2+ loss of activity in the absence of excess Fe2+ plus reducing agents Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.32 23000
-
 2 * 23000 + 2 * 27000, SDS-PAGE Pseudomonas putida
4.2.1.32 27000
-
 2 * 23000 + 2 * 27000, SDS-PAGE Pseudomonas putida
4.2.1.32 100000
-
 gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.32 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.32
-
 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.32 78
-
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.32 L-(+)-Tartrate
-
 Pseudomonas putida Oxaloacetate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.32 tetramer 2 * 23000 + 2 * 27000, SDS-PAGE Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.32 25
-
 assay at Pseudomonas putida