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Literature summary extracted from

  • Ueda, M.; Yamada, H.; Asano, Y.
    The substrate specificity of maleate hydratase from Arthrobacter sp. strain MCI2612 (1994), Appl. Microbiol. Biotechnol., 41, 215-218.
No PubMed abstract available

Application

EC Number Application Comment Organism
4.2.1.31 synthesis use for synthesis of chiral synthons considered Arthrobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.31 (2R,3S)-tartaric acid Ki: 3.15 mM Arthrobacter sp.
4.2.1.31 acetylenedicarboxylate Ki: 4.55 mM Arthrobacter sp.
4.2.1.31 additional information
-
Arthrobacter sp.
4.2.1.31 oxaloacetic acid Ki: 8.22 mM Arthrobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.31 2.74
-
2-chloromaleate
-
Arthrobacter sp.
4.2.1.31 3.27
-
2-bromomaleate
-
Arthrobacter sp.
4.2.1.31 3.85
-
Maleate
-
Arthrobacter sp.
4.2.1.31 4.55
-
citraconate
-
Arthrobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.31 28000
-
alpha1 beta1, 1 * 58000 + 1 * 28000, SDS-PAGE Arthrobacter sp.
4.2.1.31 58000
-
alpha1 beta1, 1 * 58000 + 1 * 28000, SDS-PAGE Arthrobacter sp.
4.2.1.31 90000
-
gel filtration Arthrobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.31 Arthrobacter sp.
-
-
-
4.2.1.31 Arthrobacter sp. MCI2612
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.31 bromomaleate
-
Arthrobacter sp. (-)-erythro((2S, 3S)-3-)-bromomalate
-
?
4.2.1.31 bromomaleate
-
Arthrobacter sp. MCI2612 (-)-erythro((2S, 3S)-3-)-bromomalate
-
?
4.2.1.31 chloromaleate
-
Arthrobacter sp. (-)-erythro((2S, 3S)-3-)-chloromalate
-
?
4.2.1.31 chloromaleate
-
Arthrobacter sp. MCI2612 (-)-erythro((2S, 3S)-3-)-chloromalate
-
?
4.2.1.31 citraconate
-
Arthrobacter sp. D-citramalate
-
?
4.2.1.31 citraconate
-
Arthrobacter sp. MCI2612 D-citramalate
-
?
4.2.1.31 maleate + H2O
-
Arthrobacter sp. (R)-malate
-
r
4.2.1.31 maleate + H2O
-
Arthrobacter sp. MCI2612 (R)-malate
-
r

Subunits

EC Number Subunits Comment Organism
4.2.1.31 dimer alpha1 beta1, 1 * 58000 + 1 * 28000, SDS-PAGE Arthrobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.31 45
-
-
Arthrobacter sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.31 3.15
-
(2R,3S)-tartaric acid
-
Arthrobacter sp.
4.2.1.31 4.55
-
acetylenedicarboxylate
-
Arthrobacter sp.
4.2.1.31 8.22
-
oxaloacetic acid
-
Arthrobacter sp.