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Literature summary extracted from
- Diol metabolism and diol dehydratase in Clostridium glycoliticum (1986), Arch. Biochem. Biophys., 245, 144-152.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.28 | dithiothreitol | activates, 900% stimulation at 1 mM | Terrisporobacter glycolicus |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.28 | EDTA | - |
Terrisporobacter glycolicus | |
| 4.2.1.28 | EGTA | 62% inhibition at 0.05 mM | Terrisporobacter glycolicus | |
| 4.2.1.28 | ethylene glycol bis -N,N,N,N-tetraacetic acid | beta-aminoethyl ether | Terrisporobacter glycolicus | |
| 4.2.1.28 | hydroxylamine | - |
Terrisporobacter glycolicus | |
| 4.2.1.28 | Hydroxyurea | - |
Terrisporobacter glycolicus | |
| 4.2.1.28 | iodoacetamide | complete inhibition at 5 mM | Terrisporobacter glycolicus | |
| 4.2.1.28 | o-phenanthroline | - |
Terrisporobacter glycolicus | |
| 4.2.1.28 | p-chloromercuriphenylsulfonic acid | complete inhibition at 0.1 mM | Terrisporobacter glycolicus | |
| 4.2.1.28 | sulfhydryl compounds | - |
Terrisporobacter glycolicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.28 | 3 | - |
1,2-ethanediol | - |
Terrisporobacter glycolicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.2.1.28 | cell membrane | associated with | Terrisporobacter glycolicus | - |
- |
| 4.2.1.28 | cell membrane | intergral membrane protein | Terrisporobacter glycolicus | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.28 | divalent cations | - |
Terrisporobacter glycolicus | |
| 4.2.1.28 | K+ | no requirement | Terrisporobacter glycolicus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.28 | Terrisporobacter glycolicus | - |
- |
- |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 4.2.1.28 | extremely O2 sensitive | Terrisporobacter glycolicus |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 4.2.1.28 | propane-1,2-diol = propanal + H2O | radical mechanism involved | Terrisporobacter glycolicus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.28 | additional information | - |
- |
Terrisporobacter glycolicus |
| 4.2.1.28 | 1.5 | - |
under strictly anaerobic conditions | Terrisporobacter glycolicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.28 | 1,2-ethanediol | - |
Terrisporobacter glycolicus | acetaldehyde | - |
? | |
| 4.2.1.28 | 1,2-propanediol | - |
Terrisporobacter glycolicus | propanal | - |
? | |
| 4.2.1.28 | additional information | specific for 1,2-ethanediol and 1,2-propanediol | Terrisporobacter glycolicus | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.28 | 30 | - |
enzyme assay at | Terrisporobacter glycolicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.28 | 8 | - |
- |
Terrisporobacter glycolicus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.28 | cobamide | not involved | Terrisporobacter glycolicus | |
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