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Literature summary extracted from

  • Ichikawa, Y.; Horike, Y.; Mori, N.; Hosoi, N.; Kitamoto, Y.
    Purification and properties of propanediol dehydratase from Propionibacterium freudenreichii (1985), J. Ferment. Technol., 63, 135-141.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.28 1,2-Butanediol
-
 Propionibacterium freudenreichii
4.2.1.28 1,2-cyclohexanediol
-
 Propionibacterium freudenreichii
4.2.1.28 1,2-ethanediol weak Propionibacterium freudenreichii
4.2.1.28 1,3-butanediol
-
 Propionibacterium freudenreichii
4.2.1.28 2-fluoroethanol weak Propionibacterium freudenreichii
4.2.1.28 coenzyme B12 inactivation in absence of substrate Propionibacterium freudenreichii
4.2.1.28 isopropanolamine weak Propionibacterium freudenreichii
4.2.1.28 additional information various chemicals and substrate analogues Propionibacterium freudenreichii
4.2.1.28 o-iodobenzoic acid
-
 Propionibacterium freudenreichii
4.2.1.28 p-chloromercuribenzoate 92% inhibition by 1 mM Propionibacterium freudenreichii
4.2.1.28 phenylhydrazine
-
 Propionibacterium freudenreichii
4.2.1.28 Sodium azide
-
 Propionibacterium freudenreichii
4.2.1.28 sulfhydryl compounds
-
 Propionibacterium freudenreichii
4.2.1.28 Thiourea
-
 Propionibacterium freudenreichii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.28 additional information
-
 additional information
-
 Propionibacterium freudenreichii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.28 Cs+ slight activation Propionibacterium freudenreichii
4.2.1.28 K+ requirement for monovalent cation Propionibacterium freudenreichii
4.2.1.28 Li+ slight activation Propionibacterium freudenreichii
4.2.1.28 Na+ slight activation Propionibacterium freudenreichii
4.2.1.28 NH4+
-
 Propionibacterium freudenreichii
4.2.1.28 Rb+
-
 Propionibacterium freudenreichii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.28 230000
-
 gel filtration Propionibacterium freudenreichii

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.28 Propionibacterium freudenreichii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.28
-
 Propionibacterium freudenreichii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.28 197
-
-
 Propionibacterium freudenreichii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.28 1,2-butanediol poor substrate Propionibacterium freudenreichii butyraldehyde
-
 ?
4.2.1.28 1,2-ethanediol
-
 Propionibacterium freudenreichii acetaldehyde ? ?
4.2.1.28 1,2-propanediol
-
 Propionibacterium freudenreichii propanal
-
 ?
4.2.1.28 1,3-butanediol low turnover Propionibacterium freudenreichii ?
-
 ?
4.2.1.28 2,3-butanediol poor substrate Propionibacterium freudenreichii methyl ethyl ketone
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.28 40
-
-
 Propionibacterium freudenreichii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.28 70
-
 90% loss of activity after 3 min Propionibacterium freudenreichii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.28 9.2
-
-
 Propionibacterium freudenreichii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.28 7.2 11.2
-
 Propionibacterium freudenreichii

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.28 cobamide
-
 Propionibacterium freudenreichii