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Literature summary extracted from

  • Toraya, T.; Fukui, S.
    Coenzyme B12-dependent propanediol dehydratase system. Ternary complex between apoenzyme, coenzyme, and substrate analog (1972), Biochim. Biophys. Acta, 284, 536-548.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.2.1.28 ternary complex photostable, coenzyme alone photolabile Klebsiella pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.28 2,3-Butanediol competitive inhibitor, Ki: 2.08 mM Klebsiella pneumoniae
4.2.1.28 iodoacetamide apoenzyme completely inactivated Klebsiella pneumoniae
4.2.1.28 N-ethylmaleimide apoenzyme completely inactivated Klebsiella pneumoniae
4.2.1.28 p-chloromercuribenzoate apoenzyme completely inactivated Klebsiella pneumoniae
4.2.1.28 p-hydroxymercuribenzoate ternary complex stable, apoenzyme unstable Klebsiella pneumoniae
4.2.1.28 styrene glycol competitive inhibitor, Ki: 50.2 mM Klebsiella pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.28 0.0814
-
 1,2-propanediol
-
 Klebsiella pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.28 K+ stabilizes apoenzyme-coenzyme association Klebsiella pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.28 Klebsiella pneumoniae
-
 formerly Aerobacter aerogenes
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.2.1.28 apoenzyme-coenzyme-substrate-analog complex relatively stable to O2 compared with holoenzyme, analogues: 1,2-butanediol, styrene glycol, and others to a lesser extent Klebsiella pneumoniae

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.28 propane-1,2-diol = propanal + H2O possible role of sulfhydryl groups Klebsiella pneumoniae
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.28 1,2-butanediol formation of ternary complex between apoenzyme, coenzyme and substrate analogue Klebsiella pneumoniae butyraldehyde
-
 ?
4.2.1.28 1,2-ethanediol
-
 Klebsiella pneumoniae acetaldehyde
-
 ?
4.2.1.28 1,2-propanediol
-
 Klebsiella pneumoniae propanal
-
 ?
4.2.1.28 additional information substrate stabilizes apoenzyme-coenzyme association Klebsiella pneumoniae ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.28 40
-
 apoenzyme labile above Klebsiella pneumoniae
4.2.1.28 50
-
 40% loss of activity of ternary complex after 5 min Klebsiella pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.28 cobamide
-
 Klebsiella pneumoniae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.28 50.2
-
 styrene glycol competitive inhibitor Klebsiella pneumoniae