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Literature summary extracted from

  • O'Sullivan, J.; Ettlinger, L.
    Characterization of the acetyl-CoA synthetase of Acetobacter aceti (1976), Biochim. Biophys. Acta, 450, 410-417.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.2.1.1 denatured upon freezing Acetobacter aceti

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.1 adenylate
-
 Acetobacter aceti
6.2.1.1 AMP
-
 Acetobacter aceti
6.2.1.1 diphosphate poor Acetobacter aceti
6.2.1.1 erythrose 4-phosphate
-
 Acetobacter aceti
6.2.1.1 glyceraldehyde 3-phosphate weak Acetobacter aceti
6.2.1.1 glyoxylate
-
 Acetobacter aceti
6.2.1.1 Xylulose 5-phosphate
-
 Acetobacter aceti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.1 0.104
-
 CoA
-
 Acetobacter aceti
6.2.1.1 0.25
-
 acetate
-
 Acetobacter aceti
6.2.1.1 0.36
-
 ATP
-
 Acetobacter aceti
6.2.1.1 9
-
 propanoate
-
 Acetobacter aceti

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.1 K+ absolute requirement for a monovalent cation, stimulates, Km: 14.3 mM, inhibition above 0.1 M Acetobacter aceti
6.2.1.1 Mg2+ required Acetobacter aceti
6.2.1.1 Mg2+ Km: 0.9 mM Acetobacter aceti
6.2.1.1 Mg2+ inhibition above 7 mM Acetobacter aceti
6.2.1.1 Mn2+ 5 mM, 94% of the activation relative to Mg2+ Acetobacter aceti
6.2.1.1 Na+ absolute requirement for a monovalent cation, poor activator, Km: 33 mM, no inhibition at higher concentrations Acetobacter aceti
6.2.1.1 NH4+ absolute requirement for a monovalent cation, stimulates, Km: 14.3 mM, inhibition above 0.1 M Acetobacter aceti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.1 60000
-
 gel filtration Acetobacter aceti
6.2.1.1 77000
-
 x * 77000, SDS-PAGE Acetobacter aceti

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.1 Acetobacter aceti
-
 strain ATCC 8554
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.1
-
 Acetobacter aceti

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.1 3.5
-
-
 Acetobacter aceti

Storage Stability

EC Number Storage Stability Organism
6.2.1.1 4°C, 0.1 M buffer, pH 7.5, 3 mM mercaptoethanol, stable for several weeks Acetobacter aceti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.1 ATP + acetate + CoA highly specific for ATP Acetobacter aceti AMP + diphosphate + acetyl-CoA
-
 ?
6.2.1.1 ATP + acrylate + CoA
-
 Acetobacter aceti AMP + diphosphate + acryloyl-CoA
-
 ?
6.2.1.1 ATP + butyrate + CoA no activity Acetobacter aceti AMP + diphosphate + butyryl-CoA
-
 ?
6.2.1.1 ATP + propanoate + CoA
-
 Acetobacter aceti AMP + diphosphate + propanoyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.1 monomer x * 77000, SDS-PAGE Acetobacter aceti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.1 7.6
-
-
 Acetobacter aceti