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Literature summary extracted from

  • Skovby, F.; Kraus, J.P.; Rosenberg, L.E.
    Biosynthesis and proteolytic activation of cystathionine beta-synthase in rat liver (1984), J. Biol. Chem., 25, 588-593.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.22 0.8
-
homocysteine enzyme composed of MW 48000 subunits Rattus norvegicus
4.2.1.22 4
-
L-Ser
-
Rattus norvegicus
4.2.1.22 25
-
homocysteine enzyme composed of MW 68000 subunits Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.22 48000
-
2 * 48000, proteolytically activated enzyme, derived from 4 * 68000 enzyme, SDS-PAGE Rattus norvegicus
4.2.1.22 68000
-
2 * 48000, proteolytically activated enzyme, derived from 4 * 68000 enzyme, SDS-PAGE Rattus norvegicus
4.2.1.22 68000
-
4 * 68000, enzyme from fresh liver extract, SDS-PAGE Rattus norvegicus
4.2.1.22 100000
-
aged enzyme extract, gel filtration Rattus norvegicus
4.2.1.22 250000
-
fresh enzyme extract, gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.22 L-Serine + homocysteine Rattus norvegicus
-
?
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.22 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.2.1.22 proteolytic modification the tetrameric enzyme, MW 4 * 68000, is processed to a dimeric (2 * 48000) enzyme by proteolysis, 60fold increase of activity Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.22 liver
-
Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.22 L-Serine + homocysteine
-
Rattus norvegicus Cystathionine + H2O
-
?
4.2.1.22 L-Serine + homocysteine
-
Rattus norvegicus ?
-
?

Subunits

EC Number Subunits Comment Organism
4.2.1.22 dimer 2 * 48000, proteolytically activated enzyme, derived from 4 * 68000 enzyme, SDS-PAGE Rattus norvegicus
4.2.1.22 tetramer 4 * 68000, enzyme from fresh liver extract, SDS-PAGE Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.22 pyridoxal 5'-phosphate requirement Rattus norvegicus