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Literature summary extracted from

  • Braunstein, A.E.; Goryachenkova, E.V.
    The beta-replacement-specific pyridoxal-P-dependent lyases (1984), Adv. Enzymol. Relat. Areas Mol. Biol., 56, 1-89.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.2.1.22 2-mercaptoethanol stabilizes Homo sapiens
4.2.1.22 2-mercaptoethanol stabilizes Rattus norvegicus
4.2.1.22 high protein concentration stabilizes Homo sapiens
4.2.1.22 high protein concentration stabilizes Rattus norvegicus
4.2.1.22 lyophilization inactivates Homo sapiens
4.2.1.22 lyophilization inactivates Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.22 aminooxyacetic acid
-
Homo sapiens
4.2.1.22 aminooxyacetic acid
-
Rattus norvegicus
4.2.1.22 hydroxylamine
-
Homo sapiens
4.2.1.22 hydroxylamine
-
Rattus norvegicus
4.4.1.1 cycloserine partially competitive Rattus norvegicus
4.4.1.1 Cys
-
Rattus norvegicus
4.4.1.1 cysteamine
-
Rattus norvegicus
4.4.1.1 Penicillamine
-
Rattus norvegicus
4.4.1.10 L-Ser
-
Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.22 0.59
-
homocysteine
-
Homo sapiens
4.2.1.22 1.15
-
L-Ser
-
Homo sapiens
4.2.1.22 8
-
L-Ser
-
Rattus norvegicus
4.2.1.22 18
-
homocysteine
-
Rattus norvegicus
4.2.1.22 24
-
2-mercaptoethanol
-
Rattus norvegicus
4.2.1.22 36
-
L-Cys cosubstrate 2-mercaptoethanol Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.1 45000
-
4 * 45000, SDS-PAGE Rattus norvegicus
4.4.1.1 160000
-
-
Rattus norvegicus
4.4.1.10 120000
-
-
Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.22 L-Serine + homocysteine Gallus gallus
-
?
-
?
4.2.1.22 L-Serine + homocysteine Homo sapiens
-
?
-
?
4.2.1.22 L-Serine + homocysteine Rattus norvegicus
-
?
-
?
4.4.1.10 L-Cys + sulfite Gallus gallus production of L-cysteate, the precursor of the fetal metabolite taurine ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.22 Gallus gallus
-
-
-
4.2.1.22 Homo sapiens
-
-
-
4.2.1.22 Rattus norvegicus
-
-
-
4.4.1.1 Rattus norvegicus
-
-
-
4.4.1.10 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.1
-
Rattus norvegicus
4.4.1.10
-
Gallus gallus

Reaction

EC Number Reaction Comment Organism Reaction ID
4.4.1.10 L-cysteine + sulfite = L-cysteate + hydrogen sulfide beta-replacement reaction Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.22 liver
-
Gallus gallus
-
4.2.1.22 liver
-
Homo sapiens
-
4.2.1.22 liver
-
Rattus norvegicus
-
4.4.1.1 liver
-
Rattus norvegicus
-
4.4.1.10 embryo yolk-sac Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.1 10
-
-
Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
4.4.1.1 -50°C, potassium phosphate buffer, pH 7.5, enzyme stabilized by pyridoxal 5'-phosphate, EDTA, and dithiothreitol retains full activity for several months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.22 L-Cysteine + 2-mercaptoethanol
-
Rattus norvegicus HOOC-CH(NH2)-CH2-S-CH2-CH2OH + H2S
-
?
4.2.1.22 L-Serine + homocysteine
-
Gallus gallus Cystathionine + H2O
-
?
4.2.1.22 L-Serine + homocysteine
-
Homo sapiens Cystathionine + H2O
-
?
4.2.1.22 L-Serine + homocysteine
-
Rattus norvegicus Cystathionine + H2O
-
?
4.2.1.22 L-Serine + homocysteine
-
Gallus gallus ?
-
?
4.2.1.22 L-Serine + homocysteine
-
Homo sapiens ?
-
?
4.2.1.22 L-Serine + homocysteine
-
Rattus norvegicus ?
-
?
4.4.1.1 alpha,beta-diaminopropionate beta,gamma-elimination Rattus norvegicus ?
-
?
4.4.1.1 djenkolic acid beta,gamma-elimination Rattus norvegicus ?
-
?
4.4.1.1 homocysteine beta,gamma-elimination Rattus norvegicus ?
-
?
4.4.1.1 L-Cys alpha,beta-elimination Rattus norvegicus H2S + NH3 + pyruvate
-
?
4.4.1.1 L-cystathionine + H2O beta,gamma-elimination Rattus norvegicus ?
-
?
4.4.1.1 L-cystine alpha,beta-elimination Rattus norvegicus ?
-
?
4.4.1.1 L-homoserine beta,gamma-elimination Rattus norvegicus ?
-
?
4.4.1.1 Lanthionine beta,gamma-elimination Rattus norvegicus ?
-
?
4.4.1.10 L-Cys + 2-mercaptoethanol
-
Gallus gallus S-(2-hydroxyethyl)cysteine + ?
-
?
4.4.1.10 L-Cys + 3-mercaptopropionate weak Gallus gallus ?
-
?
4.4.1.10 L-Cys + dithiothreitol
-
Gallus gallus ?
-
?
4.4.1.10 L-Cys + sulfite
-
Gallus gallus L-cysteate + H2S
-
?
4.4.1.10 L-Cys + sulfite production of L-cysteate, the precursor of the fetal metabolite taurine Gallus gallus ?
-
?
4.4.1.10 L-Cys + thioglycolate weak Gallus gallus ?
-
?

Subunits

EC Number Subunits Comment Organism
4.4.1.1 tetramer 4 * 45000, SDS-PAGE Rattus norvegicus
4.4.1.10 dimer
-
Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.22 8.4 9
-
Homo sapiens
4.4.1.10 8.7 8.8
-
Gallus gallus

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.22 pyridoxal 5'-phosphate requirement Homo sapiens
4.2.1.22 pyridoxal 5'-phosphate requirement Rattus norvegicus
4.4.1.1 pyridoxal 5'-phosphate contains 4 mol of pyridoxal 5'-phosphate per mol of enzyme Rattus norvegicus
4.4.1.10 pyridoxal 5'-phosphate dependent Gallus gallus