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Literature summary extracted from
- Overexpression and purification of separate tryptophan synthase alpha and beta subunits from Salmonella typhimurium (1992), Protein Expr. Purif., 3, 347-354.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.20 | expression in Escherichia coli | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.20 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.20 | alpha subunit | Salmonella enterica subsp. enterica serovar Typhimurium |
| 4.2.1.20 | beta subunit | Salmonella enterica subsp. enterica serovar Typhimurium |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.2.1.20 | -20°C, beta subunit, 50 mM sodium bicine buffer, pH 7.8, 1 mM EDTA, 0.04 mM pyridoxal 5'-phoshate, 2 mM DTT | Salmonella enterica subsp. enterica serovar Typhimurium |
| 4.2.1.20 | -75°C, or liquid N2, alpha subunit | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.20 | indole + L-serine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | L-tryptophan + H2O | - |
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Release 2023.1 (January 2023)
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