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Literature summary extracted from
- Production of a functional eukaryotic enzyme in Escherichia coli: cloning and expression of the yeast structural gene for imidazoleglycerolphoshate dehydratase (his3) (1977), Proc. Natl. Acad. Sci. USA, 74, 5255-5259.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.19 | expression in Escherichia coli | Saccharomyces cerevisiae |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.19 | 3-Amino-1,2,4-triazole | Ki: 0.03 mM | Saccharomyces cerevisiae |  |
| 4.2.1.19 | phosphate | Ki: 0.008 mM | Saccharomyces cerevisiae | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.19 | 0.2 | - |
D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate | wild-type enzyme | Saccharomyces cerevisiae | |
| 4.2.1.19 | 0.3 | - |
D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate | expressed in E. coli | Saccharomyces cerevisiae | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.19 | Mn2+ | activity dependent on | Saccharomyces cerevisiae | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.19 | Saccharomyces cerevisiae | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.19 | D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate | - |
Saccharomyces cerevisiae | 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.19 | 7.5 | - |
wild-type enzyme | Saccharomyces cerevisiae |
| 4.2.1.19 | 8 | - |
enzyme expressed in Escherichia coli | Saccharomyces cerevisiae |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.19 | 6.8 | 8 | wild-type enzyme, less than 50% of maximal activity above and below | Saccharomyces cerevisiae |
| 4.2.1.19 | 7.2 | 8.2 | enzyme expressed in E. coli, less than 50% of maximal activity above and below | Saccharomyces cerevisiae |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.19 | 0.008 | - |
phosphate | - |
Saccharomyces cerevisiae | |
| 4.2.1.19 | 0.03 | - |
3-Amino-1,2,4-triazole | - |
Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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