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Literature summary extracted from

  • Staples, M.A.; Houston, L.L.
    Purification of the bifunctional enzyme imidazoleglycerolphosphate dehydratase-histidinol phosphatase of Salmonella typhimurium (1980), Biochim. Biophys. Acta, 613, 210-219.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.15 0.15
-
 L-histidinol phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.15 46000
-
 Mn2+ precipitiation, antibody affinity chromatography, selective proteolysis Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.19 46000
-
 x * 46000, bifunctional enzyme with EC 3.1.3.15, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.19 260000
-
 bifunctional enzyme with EC 3.1.3.15, gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.15 L-histidinol phosphate + H2O Salmonella enterica subsp. enterica serovar Typhimurium
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.15 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
4.2.1.19 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.15
-
 Salmonella enterica subsp. enterica serovar Typhimurium
4.2.1.19
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.15 202000
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.15 L-histidinol phosphate + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium L-histidinol + phosphate
-
 ?
3.1.3.15 L-histidinol phosphate + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
4.2.1.19 D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.19 polymer x * 46000, bifunctional enzyme with EC 3.1.3.15, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.1.19 7
-
 irreversible inactivation below Salmonella enterica subsp. enterica serovar Typhimurium