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Literature summary extracted from

  • Uchiyama, H.; Ando, M.; Toyonaka, Y.; Tabuchi, T.
    Subcellular localization of the methylcitric-acid-cycle enzymes in propionate metabolism of Yarrowia lipolytica (1982), Eur. J. Biochem., 125, 523-527.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.3.5 mitochondrion
-
 Yarrowia lipolytica 5739
-
4.1.3.30 cytoplasm
-
 Yarrowia lipolytica 5737
-
4.1.3.30 mitochondrion
-
 Yarrowia lipolytica 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.5 propanoyl-CoA + H2O + oxaloacetate Yarrowia lipolytica first key enzyme of the methylcitric acid cycle 2-methylcitrate + CoA
-
 ?
4.1.3.30 (2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate Yarrowia lipolytica the enzyme may function to supply pyruvate, the end-product of the catabolism of the propionate residue, to various reactions ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.5 Yarrowia lipolytica
-
 IFO 1659
-
4.1.3.30 Yarrowia lipolytica
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.5 0.0051
-
 nuclei-free homogenate, glucose grown cells Yarrowia lipolytica
2.3.3.5 0.0092
-
 nuclei-free homogenate, n-alkane grown cells Yarrowia lipolytica
2.3.3.5 0.019
-
 heavy particulate fraction, glucose grown cells Yarrowia lipolytica
2.3.3.5 0.03
-
 heavy particulate fraction, n-alkane grown cells Yarrowia lipolytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.5 additional information constitutive Yarrowia lipolytica ?
-
 ?
2.3.3.5 propanoyl-CoA + H2O + oxaloacetate
-
 Yarrowia lipolytica 2-methylcitrate + CoA
-
 ?
2.3.3.5 propanoyl-CoA + H2O + oxaloacetate first key enzyme of the methylcitric acid cycle Yarrowia lipolytica 2-methylcitrate + CoA
-
 ?
4.1.3.30 (2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate
-
 Yarrowia lipolytica Pyruvate + succinate
-
 ?
4.1.3.30 (2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate the enzyme may function to supply pyruvate, the end-product of the catabolism of the propionate residue, to various reactions Yarrowia lipolytica ?
-
 ?