Literature summary extracted from

Grady, S.R.; Wang, J.K.; Dekker, E.E.
Steady-state kinetics and inhibition studies of the aldol condensation reaction catalized by bovine liver and Escherichia coli 2-keto-4-hydroxyglutarate aldolase (1981), Biochemistry, 20, 2497-2502.

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
4.1.3.16	KCl	activates	Bos taurus
4.1.3.16	additional information	carboxylic acids activate	Bos taurus

EC Number	Inhibitors	Comment	Organism
4.1.3.16	2-Ketoglutarate	12% inhibition at 20 mM	Bos taurus
4.1.3.16	acetaldehyde	-	Bos taurus
4.1.3.16	chloride	41% inhibition at 40 mM	Bos taurus
4.1.3.16	citrate	-	Bos taurus
4.1.3.16	Glyoxal	36% inhibition at 20 mM	Bos taurus
4.1.3.16	glyoxylate	bovine enzyme inhibited at lower concentrations than E. coli enzyme	Bos taurus
4.1.3.16	Halides	-	Bos taurus
4.1.3.16	Hydroxypyruvate	91% inhibition at 20 mM	Bos taurus
4.1.3.16	additional information	-	Bos taurus
4.1.3.16	NaBr	-	Bos taurus
4.1.3.16	NaCl	-	Bos taurus
4.1.3.16	NaF	-	Bos taurus
4.1.3.16	NaI	-	Bos taurus
4.1.3.42	2-Ketoglutarate	-	Escherichia coli
4.1.3.42	acetaldehyde	Ki: 11 mM	Escherichia coli
4.1.3.42	chloride	41% inhibition at 40 mM	Escherichia coli
4.1.3.42	citrate	Ki: 7.5 mM	Escherichia coli
4.1.3.42	glyoxylate	bovine enzyme inhibited at lower concentrations than E. coli enzyme	Escherichia coli
4.1.3.42	Halides	-	Escherichia coli
4.1.3.42	Hydroxypyruvate	Ki: 0.0125 mM	Escherichia coli
4.1.3.42	additional information	-	Escherichia coli
4.1.3.42	NaBr	48% inhibition at 20 mM	Escherichia coli
4.1.3.42	NaCl	38% inhibition at 20 mM	Escherichia coli
4.1.3.42	NaF	19% inhibition at 20 mM	Escherichia coli
4.1.3.42	NaI	59% inhibition at 20 mM	Escherichia coli

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
4.1.3.16	additional information	-	additional information	-	Bos taurus
4.1.3.42	additional information	-	additional information	-	Escherichia coli
4.1.3.42	14.3	-	glyoxylate	-	Escherichia coli
4.1.3.42	45	-	pyruvate	-	Escherichia coli

EC Number	Metals/Ions	Comment	Organism	Structure
4.1.3.16	additional information	no requirement	Bos taurus
4.1.3.42	additional information	no requirement	Escherichia coli

EC Number	Organism	UniProt	Comment	Textmining
4.1.3.16	Bos taurus	-	bovine	-
4.1.3.42	Escherichia coli	-	-	-

EC Number	Source Tissue	Comment	Organism	Textmining

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4.1.3.16	additional information	mechanism	Bos taurus	?	-	?
4.1.3.42	additional information	mechanism	Escherichia coli	?	-	?

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
4.1.3.42	0.0125	-	Hydroxypyruvate	-	Escherichia coli
4.1.3.42	7.5	-	citrate	-	Escherichia coli