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Literature summary extracted from

  • Lane, R.S.; Shapley, A.; Dekker, E.E.
    2-keto-4-Hydroxybutyrate aldolase. Identification as 2-keto-4-hydroxyglutarate aldolase, catalytic properties, and role in mammalian metabolism of L-homoserine (1971), Biochemistry, 10, 1353-1364.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.16 2-Ketobutyrate competitive, Ki for 2-keto-4-hydroxybutyrate cleavage: 53 mM, Ki for 2-keto-4-hydroxyglutarate cleavage: 52 mM Bos taurus
4.1.3.16 2-Ketoglutarate competitive, Ki for 2-keto-4-hydroxybutyrate cleavage: 18 mM, Ki for 2-keto-4-hydroxyglutarate cleavage: 18 mM Bos taurus
4.1.3.16 glycolaldehyde competitive, Ki for 2-keto-4-hydroxybutyrate cleavage: 2.7 mM, Ki for 2-keto-4-hydroxyglutarate cleavage: 2.4 mM Bos taurus
4.1.3.16 NaBH4
-
 Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.16 0.1
-
 4-hydroxy-alpha-ketoglutarate
-
 Bos taurus
4.1.3.16 3.1
-
 2-Keto-4-hydroxybutyrate
-
 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.16 additional information no requirement Mammalia
4.1.3.16 additional information no requirement Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.16 2-Keto-4-hydroxybutyrate Bos taurus metabolism of L-homoserine ?
-
 ?
4.1.3.16 4-hydroxy-2-oxoglutarate Mammalia catabolism of hydroxyproline, condensation physiologically less important ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.16 Bos taurus
-
 bovine
-
4.1.3.16 Mammalia
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.16
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.3.16 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.16 0.53
-
 2-keto-4-hydroxybutyrate Bos taurus
4.1.3.16 1.8
-
 2-keto-4-hydroxyglutarate Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.16 2-Keto-4-hydroxybutyrate D-isomer favoured Bos taurus Pyruvate + formaldehyde
-
 ?
4.1.3.16 2-Keto-4-hydroxybutyrate metabolism of L-homoserine Bos taurus ?
-
 ?
4.1.3.16 4-Hydroxy-2-oxoglutarate
-
 Bos taurus Pyruvate + glyoxylate
-
 ?
4.1.3.16 4-hydroxy-2-oxoglutarate catabolism of hydroxyproline, condensation physiologically less important Mammalia ?
-
 ?
4.1.3.16 additional information mechanism Bos taurus ?
-
 ?
4.1.3.16 Pyruvate + formaldehyde Keq: 4.1 mM Bos taurus 2-Keto-4-hydroxybutyrate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.3.16 37
-
 enzyme assay at Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.3.16 59
-
 constant decay of 0.011 1/min, slow inactivation Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.16 8.1
-
 2-keto-4-hydroxybutyrate cleavage Bos taurus
4.1.3.16 8.8
-
 2-keto-4-hydroxyglutarate cleavage Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.3.16 6 9.5 2-keto-4-hydroxybutyrate cleavage Bos taurus
4.1.3.16 7 9 2-keto-4-hydroxyglutarate cleavage Bos taurus