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Literature summary extracted from

  • Crow, V.L.; Thomas, T.D.
    D-Tagatose 1,6-diphosphate aldolase from lactic Streptococci: purification, properties, and use in measuring intracellular tagatose 1,6-diphosphate (1982), J. Bacteriol., 151, 600-608.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
4.1.2.40 analysis use in measuring intracellular tagatose 1,6-diphosphate Lactococcus cremoris

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.40 EDTA slight Lactococcus cremoris
4.1.2.40 NaBH4 substrate-dependent inactivation Lactococcus cremoris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.40 0.1
-
 tagatose 1,6-diphosphate
-
 Lactococcus cremoris
4.1.2.40 0.25
-
 fructose 1,6-diphosphate
-
 Lactococcus cremoris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.40 D-Tagatose-1,6-bisphosphate Lactococcus lactis induced by growth on either lactose or galactose ?
-
 ?
4.1.2.40 D-Tagatose-1,6-bisphosphate Lactococcus cremoris induced by growth on either lactose or galactose ?
-
 ?
4.1.2.40 D-Tagatose-1,6-bisphosphate Lactococcus lactis C10 induced by growth on either lactose or galactose ?
-
 ?
4.1.2.40 D-Tagatose-1,6-bisphosphate Lactococcus cremoris E8 induced by growth on either lactose or galactose ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.40 Lactococcus cremoris
-
-
-
4.1.2.40 Lactococcus cremoris E8
-
-
-
4.1.2.40 Lactococcus lactis
-
-
-
4.1.2.40 Lactococcus lactis C10
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.40
-
 Lactococcus cremoris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.40 32.2
-
-
 Lactococcus cremoris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.40 D-Fructose 1,6-diphosphate
-
 Lactococcus lactis Glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.40 D-Fructose 1,6-diphosphate
-
 Lactococcus cremoris Glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.40 D-Fructose 1,6-diphosphate
-
 Lactococcus lactis C10 Glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.40 D-Fructose 1,6-diphosphate
-
 Lactococcus cremoris E8 Glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.40 D-Tagatose-1,6-bisphosphate
-
 Lactococcus lactis Glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.40 D-Tagatose-1,6-bisphosphate
-
 Lactococcus cremoris Glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.40 D-Tagatose-1,6-bisphosphate
-
 Lactococcus lactis C10 Glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.40 D-Tagatose-1,6-bisphosphate
-
 Lactococcus cremoris E8 Glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.40 D-Tagatose-1,6-bisphosphate induced by growth on either lactose or galactose Lactococcus lactis ?
-
 ?
4.1.2.40 D-Tagatose-1,6-bisphosphate induced by growth on either lactose or galactose Lactococcus cremoris ?
-
 ?
4.1.2.40 D-Tagatose-1,6-bisphosphate induced by growth on either lactose or galactose Lactococcus lactis C10 ?
-
 ?
4.1.2.40 D-Tagatose-1,6-bisphosphate induced by growth on either lactose or galactose Lactococcus cremoris E8 ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.40 7 7.3 with tagatose 1,6-diphosphate or fructose 1,6-diphosphate as substrate Lactococcus cremoris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.2.40 6.6 7.6 85% of maximal activity at pH 6.6 and at pH 7.6, with tagatose 1,6-diphosphate or fructose 1,6-diphosphate as substrate Lactococcus cremoris