EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
6.1.1.7 | C290S | mutant enzymes with replacement of Cys residues, Cys76Ser, Cys290Ser, Cys412Ser, Cys665Ser. Mutation of Cys665 to serine induces a 120-fold decrease in catalytic efficiency | Escherichia coli |
6.1.1.7 | C665S | mutant enzymes with replacement of Cys residues, Cys76Ser, Cys290Ser, Cys412Ser, Cys665Ser. Mutation of Cys665 to serine induces a 120-fold decrease in catalytic efficiency | Escherichia coli |
6.1.1.7 | C76S | mutant enzymes with replacement of Cys residues, Cys76Ser, Cys290Ser, Cys412Ser, Cys665Ser. Mutation of Cys665 to serine induces a 120-fold decrease in catalytic efficiency | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.7 | 5,5'-dithiobis(2-nitrobenzoate) | - |
Escherichia coli | |
6.1.1.7 | monobromobimane | - |
Escherichia coli | |
6.1.1.7 | p-Hydroxymercuriphenylsulfonic acid | - |
Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.7 | 0.083 | - |
ATP | wild-type enzyme | Escherichia coli | |
6.1.1.7 | 0.095 | - |
ATP | Cys665Ser mtant | Escherichia coli | |
6.1.1.7 | 0.12 | - |
ATP | Cys412Ser mutant | Escherichia coli | |
6.1.1.7 | 0.18 | - |
ATP | Cys76Ser mutant | Escherichia coli | |
6.1.1.7 | 0.21 | - |
ATP | Cys290Ser mutant | Escherichia coli | |
6.1.1.7 | 0.23 | - |
alanine | Cys665Ser mutant | Escherichia coli | |
6.1.1.7 | 0.33 | - |
alanine | mutant Cys76Ser | Escherichia coli | |
6.1.1.7 | 0.34 | - |
alanine | wild-type | Escherichia coli | |
6.1.1.7 | 0.47 | - |
alanine | Cys412Ser mutant | Escherichia coli | |
6.1.1.7 | 0.5 | - |
alanine | Cys290Ser mutant | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.7 | Zinc | thightly bound | Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.7 | Escherichia coli | - |
wild-type and mutant enzymes with replacement ofcysteine residues, Cys76Ser, Cys290Ser, Cys412Ser, Cys665Ser | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.7 | ATP + L-alanine + tRNAAla | - |
Escherichia coli | AMP + diphosphate + L-alanyl-tRNAAla | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
6.1.1.7 | 79 | - |
ATP | Cys412Ser mutant | Escherichia coli | |
6.1.1.7 | 84 | - |
ATP | wild-type | Escherichia coli | |
6.1.1.7 | 89 | - |
ATP | Cys665Ser mutant | Escherichia coli | |
6.1.1.7 | 110 | - |
ATP | Cys290Ser mutant | Escherichia coli | |
6.1.1.7 | 120 | - |
ATP | Cys76Ser mutant | Escherichia coli |