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Literature summary extracted from
- Kinetic analysis of duodenal and testicular cytochrome P450c17 in the rat (1996), J. Steroid Biochem. Mol. Biol., 58, 577-584.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.19 | estradiol-17beta | inhibits both and C17,20 lyase activity in testis and duodenum, competitive inhibitor | Rattus norvegicus |  |
| 1.14.14.32 | estradiol-17beta | concentration of 0.3 mM both, testicular and male duodenal enzyme with exogenous 17alpha-hydroxyprogesterone as substrate are inhibited by 30-40% | Rattus norvegicus | |
| 1.14.14.32 | ketoconazole | competitve | Rattus norvegicus | |
| 1.14.14.32 | Promegestone | competitive | Rattus norvegicus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.14.19 | 0.0000142 | - |
progesterone | 17alpha-hydroxylation in testis | Rattus norvegicus | |
| 1.14.14.19 | 0.0000232 | - |
progesterone | 17alpha-hydroxylation in female duodenum | Rattus norvegicus | |
| 1.14.14.19 | 0.0000238 | - |
progesterone | 17alpha-hydroxylation in male duodenum | Rattus norvegicus | |
| 1.14.14.19 | 0.000525 | - |
17alpha-hydroxyprogesterone | C17,20 lyase activity in testis | Rattus norvegicus | |
| 1.14.14.19 | 0.000637 | - |
17alpha-hydroxyprogesterone | C17,20 lyase activity in female duodenum | Rattus norvegicus | |
| 1.14.14.19 | 0.000675 | - |
17alpha-hydroxyprogesterone | C17,20 lyase activity in male duodenum | Rattus norvegicus | |
| 1.14.14.32 | 0.000015 | - |
17alpha-hydroxyprogesterone | intermediate substrate, testis enzyme | Rattus norvegicus | |
| 1.14.14.32 | 0.0000248 | - |
17alpha-hydroxyprogesterone | intermediate substrate, female duodenum enzyme | Rattus norvegicus | |
| 1.14.14.32 | 0.0000314 | - |
17alpha-hydroxyprogesterone | intermediate substrate, male duodenum enzyme | Rattus norvegicus | |
| 1.14.14.32 | 0.000525 | - |
17alpha-hydroxyprogesterone | exogenous substrate, testis enzyme | Rattus norvegicus | |
| 1.14.14.32 | 0.000637 | - |
17alpha-hydroxyprogesterone | exogenous substrate, female duodenum enzyme | Rattus norvegicus | |
| 1.14.14.32 | 0.000675 | - |
17alpha-hydroxyprogesterone | exogenous substrate, male duodenum enzyme | Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.14.19 | microsome | - |
Rattus norvegicus | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.19 | Fe3+ | - |
Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.19 | pregnenolone + AH2 + O2 | Rattus norvegicus | - |
17alpha-hydroxypregnenolone + A + H2O | product eliminates at C20,21 acetate to yield dehydroepiandrosterone | ? | |
| 1.14.14.19 | progesterone + [reduced NADPH-hemoprotein reductase] + O2 | Rattus norvegicus | - |
17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O | product eliminates at C20,21 acetate to yield androstenedione | ? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.19 | Rattus norvegicus | - |
- |
- |
| 1.14.14.32 | Rattus norvegicus | - |
Wistar rat | - |
| 1.14.14.32 | vertebrata | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.14.19 | duodenum | - |
Rattus norvegicus | - |
| 1.14.14.19 | testis | - |
Rattus norvegicus | - |
| 1.14.14.32 | duodenum | - |
Rattus norvegicus | - |
| 1.14.14.32 | gonad | - |
Rattus norvegicus | - |
| 1.14.14.32 | gonad | - |
vertebrata | - |
| 1.14.14.32 | liver | - |
Rattus norvegicus | - |
| 1.14.14.32 | placenta | - |
Rattus norvegicus | - |
| 1.14.14.32 | stomach | - |
Rattus norvegicus | - |
| 1.14.14.32 | testis | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.19 | pregnenolone + AH2 + O2 | - |
Rattus norvegicus | 17alpha-hydroxypregnenolone + A + H2O | product eliminates at C20,21 acetate to yield dehydroepiandrosterone | ? | |
| 1.14.14.19 | progesterone + [reduced NADPH-hemoprotein reductase] + O2 | - |
Rattus norvegicus | 17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O | product eliminates at C20,21 acetate to yield androstenedione | ? | |
| 1.14.14.32 | 17alpha-Hydroxypregnenolone | - |
Rattus norvegicus | Dehydroepiandrosterone + acetaldehyde | - |
? | |
| 1.14.14.32 | 17alpha-hydroxyprogesterone + [reduced NADPH-hemoprotein reductase] + O2 | - |
Rattus norvegicus | androstenedione + acetate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.19 | 37 | - |
- |
Rattus norvegicus |
| 1.14.14.32 | 37 | - |
assay at | Rattus norvegicus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.19 | NADPH | - |
Rattus norvegicus | |
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