EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.92 | additional information | enzyme is induced by growth on myo-inositol | Klebsiella aerogenes | |
4.1.2.29 | additional information | no requirement | Klebsiella aerogenes |
EC Number | General Stability | Organism |
---|---|---|
2.7.1.92 | purified enzyme is unstable to repeated freezing and thawing in 20 mM potassium phosphate buffer, pH 7, 5 mM EDTA, 10 mM 2-mercaptoethanol | Klebsiella aerogenes |
4.1.2.29 | resistant to repeated freezing and thawing | Klebsiella aerogenes |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.2.29 | NaBH4 | - |
Klebsiella aerogenes |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.92 | 0.097 | - |
2-deoxy-5-keto-D-gluconic acid | - |
Klebsiella aerogenes | |
2.7.1.92 | 0.22 | - |
ATP | - |
Klebsiella aerogenes | |
4.1.2.29 | 0.037 | - |
6-phospho-5-dehydro-2-deoxy-D-gluconate | - |
Klebsiella aerogenes |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.92 | Mg2+ | MgCl2 is included in assay mixture on the assumption that it is required | Klebsiella aerogenes |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.92 | 225000 | - |
sucrose density gradient centrifugation | Klebsiella aerogenes |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.92 | ATP + 2-deoxy-5-keto-D-gluconic acid | Klebsiella aerogenes | involved in myo-inositol degradation | ADP + 2-deoxy-5-keto-D-gluconic acid 6-phosphate | - |
? | |
2.7.1.92 | ATP + 2-deoxy-5-keto-D-gluconic acid | Klebsiella aerogenes constitutive | involved in myo-inositol degradation | ADP + 2-deoxy-5-keto-D-gluconic acid 6-phosphate | - |
? | |
4.1.2.29 | 6-Phospho-5-dehydro-2-deoxy-D-gluconate | Klebsiella aerogenes | enzyme is involved in myo-inositol catabolism | ? | - |
? | |
4.1.2.29 | 6-Phospho-5-dehydro-2-deoxy-D-gluconate | Klebsiella aerogenes constitutive | enzyme is involved in myo-inositol catabolism | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.92 | Klebsiella aerogenes | - |
C1, constitutive strain | - |
2.7.1.92 | Klebsiella aerogenes constitutive | - |
C1, constitutive strain | - |
4.1.2.29 | Klebsiella aerogenes | - |
- |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
4.1.2.29 | inactivation by reduction with NaBH4 in presence of dihydroxyaceton phosphate | Klebsiella aerogenes |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.92 | partial, 20fold, not seperated from 2-deoxy-5-keto-D-gluconic acid 6-phosphate aldolase | Klebsiella aerogenes |
4.1.2.29 | - |
Klebsiella aerogenes |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.92 | 13.6 | - |
- |
Klebsiella aerogenes |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.1.92 | 0°C, 20 mM potassium phosphate buffer, pH 7, 5 mM EDTA, 10 mM 2-mercaptoethanol, 1 month or more, stable | Klebsiella aerogenes |
2.7.1.92 | 4°C, enzyme of DEAE-Sephadex fractions, 20 mM potassium phosphate buffer, pH 7, 5 mM EDTA, 10 mM 2-mercaptoethanol, 0.2 M KCl, 5 months, stable | Klebsiella aerogenes |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.92 | ATP + 2-deoxy-5-keto-D-gluconic acid | - |
Klebsiella aerogenes | ADP + 2-deoxy-5-keto-D-gluconic acid 6-phosphate | - |
? | |
2.7.1.92 | ATP + 2-deoxy-5-keto-D-gluconic acid | involved in myo-inositol degradation | Klebsiella aerogenes | ADP + 2-deoxy-5-keto-D-gluconic acid 6-phosphate | - |
? | |
2.7.1.92 | ATP + 2-deoxy-5-keto-D-gluconic acid | - |
Klebsiella aerogenes constitutive | ADP + 2-deoxy-5-keto-D-gluconic acid 6-phosphate | - |
? | |
2.7.1.92 | ATP + 2-deoxy-5-keto-D-gluconic acid | involved in myo-inositol degradation | Klebsiella aerogenes constitutive | ADP + 2-deoxy-5-keto-D-gluconic acid 6-phosphate | - |
? | |
4.1.2.29 | 6-phospho-5-dehydro-2-deoxy-D-gluconate | - |
Klebsiella aerogenes | glycerone phosphate + malonate semialdehyde | - |
? | |
4.1.2.29 | 6-phospho-5-dehydro-2-deoxy-D-gluconate | - |
Klebsiella aerogenes constitutive | glycerone phosphate + malonate semialdehyde | - |
? | |
4.1.2.29 | 6-Phospho-5-dehydro-2-deoxy-D-gluconate | enzyme is involved in myo-inositol catabolism | Klebsiella aerogenes | ? | - |
? | |
4.1.2.29 | 6-Phospho-5-dehydro-2-deoxy-D-gluconate | enzyme is involved in myo-inositol catabolism | Klebsiella aerogenes constitutive | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.92 | 30 | - |
assay at | Klebsiella aerogenes |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.92 | 7 | 8.5 | broad, Tris-chloride buffer | Klebsiella aerogenes |
2.7.1.92 | 8 | - |
assay at | Klebsiella aerogenes |
4.1.2.29 | 7 | 8.7 | - |
Klebsiella aerogenes |