Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Anderson, W.A.; Magasanik, B.
    The pathway of myo-inositol degradation in Aerobacter aerogenes. Conversion of 2-deoxy-5-keto-D-gluconic acid to glycolytic intermediates (1971), J. Biol. Chem., 246, 5662-5675.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.92 additional information enzyme is induced by growth on myo-inositol Klebsiella aerogenes
4.1.2.29 additional information no requirement Klebsiella aerogenes

General Stability

EC Number General Stability Organism
2.7.1.92 purified enzyme is unstable to repeated freezing and thawing in 20 mM potassium phosphate buffer, pH 7, 5 mM EDTA, 10 mM 2-mercaptoethanol Klebsiella aerogenes
4.1.2.29 resistant to repeated freezing and thawing Klebsiella aerogenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.29 NaBH4
-
Klebsiella aerogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.92 0.097
-
2-deoxy-5-keto-D-gluconic acid
-
Klebsiella aerogenes
2.7.1.92 0.22
-
ATP
-
Klebsiella aerogenes
4.1.2.29 0.037
-
6-phospho-5-dehydro-2-deoxy-D-gluconate
-
Klebsiella aerogenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.92 Mg2+ MgCl2 is included in assay mixture on the assumption that it is required Klebsiella aerogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.92 225000
-
sucrose density gradient centrifugation Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.92 ATP + 2-deoxy-5-keto-D-gluconic acid Klebsiella aerogenes involved in myo-inositol degradation ADP + 2-deoxy-5-keto-D-gluconic acid 6-phosphate
-
?
2.7.1.92 ATP + 2-deoxy-5-keto-D-gluconic acid Klebsiella aerogenes constitutive involved in myo-inositol degradation ADP + 2-deoxy-5-keto-D-gluconic acid 6-phosphate
-
?
4.1.2.29 6-Phospho-5-dehydro-2-deoxy-D-gluconate Klebsiella aerogenes enzyme is involved in myo-inositol catabolism ?
-
?
4.1.2.29 6-Phospho-5-dehydro-2-deoxy-D-gluconate Klebsiella aerogenes constitutive enzyme is involved in myo-inositol catabolism ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.92 Klebsiella aerogenes
-
C1, constitutive strain
-
2.7.1.92 Klebsiella aerogenes constitutive
-
C1, constitutive strain
-
4.1.2.29 Klebsiella aerogenes
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.1.2.29 inactivation by reduction with NaBH4 in presence of dihydroxyaceton phosphate Klebsiella aerogenes

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.92 partial, 20fold, not seperated from 2-deoxy-5-keto-D-gluconic acid 6-phosphate aldolase Klebsiella aerogenes
4.1.2.29
-
Klebsiella aerogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.92 13.6
-
-
Klebsiella aerogenes

Storage Stability

EC Number Storage Stability Organism
2.7.1.92 0°C, 20 mM potassium phosphate buffer, pH 7, 5 mM EDTA, 10 mM 2-mercaptoethanol, 1 month or more, stable Klebsiella aerogenes
2.7.1.92 4°C, enzyme of DEAE-Sephadex fractions, 20 mM potassium phosphate buffer, pH 7, 5 mM EDTA, 10 mM 2-mercaptoethanol, 0.2 M KCl, 5 months, stable Klebsiella aerogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.92 ATP + 2-deoxy-5-keto-D-gluconic acid
-
Klebsiella aerogenes ADP + 2-deoxy-5-keto-D-gluconic acid 6-phosphate
-
?
2.7.1.92 ATP + 2-deoxy-5-keto-D-gluconic acid involved in myo-inositol degradation Klebsiella aerogenes ADP + 2-deoxy-5-keto-D-gluconic acid 6-phosphate
-
?
2.7.1.92 ATP + 2-deoxy-5-keto-D-gluconic acid
-
Klebsiella aerogenes constitutive ADP + 2-deoxy-5-keto-D-gluconic acid 6-phosphate
-
?
2.7.1.92 ATP + 2-deoxy-5-keto-D-gluconic acid involved in myo-inositol degradation Klebsiella aerogenes constitutive ADP + 2-deoxy-5-keto-D-gluconic acid 6-phosphate
-
?
4.1.2.29 6-phospho-5-dehydro-2-deoxy-D-gluconate
-
Klebsiella aerogenes glycerone phosphate + malonate semialdehyde
-
?
4.1.2.29 6-phospho-5-dehydro-2-deoxy-D-gluconate
-
Klebsiella aerogenes constitutive glycerone phosphate + malonate semialdehyde
-
?
4.1.2.29 6-Phospho-5-dehydro-2-deoxy-D-gluconate enzyme is involved in myo-inositol catabolism Klebsiella aerogenes ?
-
?
4.1.2.29 6-Phospho-5-dehydro-2-deoxy-D-gluconate enzyme is involved in myo-inositol catabolism Klebsiella aerogenes constitutive ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.92 30
-
assay at Klebsiella aerogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.92 7 8.5 broad, Tris-chloride buffer Klebsiella aerogenes
2.7.1.92 8
-
assay at Klebsiella aerogenes
4.1.2.29 7 8.7
-
Klebsiella aerogenes