Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Dahms, A.S.; Anderson, R.L.
    D-Fucose metabolism in a pseudomonad. IV. Cleavage of 2-keto-3-deoxy-D-fuconate to pyruvate and D-lactaldehyde by 2-keto-3-deoxy-L-arabonate aldolase (1972), J. Biol. Chem., 247, 2238-2241.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.1.2.18 thiols stabilize during purification Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.18 1.8
-
 2-Keto-3-deoxy-L-arabonate
-
 Pseudomonas sp.
4.1.2.18 2.9
-
 2-Keto-3-deoxy-D-fuconate
-
 Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.18 Co2+ divalent cation required Pseudomonas sp.
4.1.2.18 Co2+ 95% of the activation with Mn2+ Pseudomonas sp.
4.1.2.18 Mg2+ divalent cation required Pseudomonas sp.
4.1.2.18 Mg2+ 54% of the activity with Mn2+ Pseudomonas sp.
4.1.2.18 Mn2+ divalent cation required Pseudomonas sp.
4.1.2.18 Mn2+ most effective Pseudomonas sp.
4.1.2.18 Ni2+ divalent cation required Pseudomonas sp.
4.1.2.18 Ni2+ 45% of the activity with Mn2+ Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.18 additional information Pseudomonas sp. enzyme activity increases 3fold by growth on D-fucose or L-arabinose ?
-
 ?
4.1.2.18 additional information Pseudomonas sp. MSU-1 enzyme activity increases 3fold by growth on D-fucose or L-arabinose ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.18 Pseudomonas sp.
-
-
-
4.1.2.18 Pseudomonas sp. MSU-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.18
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.18 1.63
-
-
 Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
4.1.2.18 -20°C, 0.1 M bicine, 0.14 mM 2-thioethanol, 3 weeks, 70% loss of activity Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.18 2-Keto-3-deoxy-D-fuconate
-
 Pseudomonas sp. Pyruvate + D-lactaldehyde
-
 ?
4.1.2.18 2-Keto-3-deoxy-D-fuconate
-
 Pseudomonas sp. MSU-1 Pyruvate + D-lactaldehyde
-
 ?
4.1.2.18 2-Keto-3-deoxy-L-arabonate
-
 Pseudomonas sp. Pyruvate + glycolaldehyde
-
 ?
4.1.2.18 2-Keto-3-deoxy-L-arabonate
-
 Pseudomonas sp. MSU-1 Pyruvate + glycolaldehyde
-
 ?
4.1.2.18 additional information enzyme activity increases 3fold by growth on D-fucose or L-arabinose Pseudomonas sp. ?
-
 ?
4.1.2.18 additional information enzyme activity increases 3fold by growth on D-fucose or L-arabinose Pseudomonas sp. MSU-1 ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.2.18 55
-
 half-life: 15 min Pseudomonas sp.