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Literature summary extracted from

  • Jayanthi Bai, N.; Ramachandra Pai, M.; Suryanarayana Murthy, P.; Venkitasubramanian, T.A.
    Fructose-bisphosphate aldolases from Mycobacteria (1982), Methods Enzymol., 90, 241-250.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.1.2.13 carboxypeptidase A treatment results in 20% inactivation. Complete protection by D-fructose 1,6-bisphosphate Mycolicibacterium smegmatis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.13 1,10-phenanthroline aldolase class II Mycobacterium tuberculosis
4.1.2.13 2,2'-dipyridyl aldolase class II Mycobacterium tuberculosis
4.1.2.13 8-hydroxyquinoline aldolase class II Mycobacterium tuberculosis
4.1.2.13 Co2+
-
 Mycolicibacterium smegmatis
4.1.2.13 Cu2+
-
 Mycolicibacterium smegmatis
4.1.2.13 EDTA
-
 Mycobacterium tuberculosis
4.1.2.13 EDTA insensitive Mycolicibacterium smegmatis
4.1.2.13 Fe2+
-
 Mycolicibacterium smegmatis
4.1.2.13 Mn2+
-
 Mycolicibacterium smegmatis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.13 0.002
-
 D-fructose 1,6-bisphosphate
-
 Mycolicibacterium smegmatis
4.1.2.13 0.08
-
 D-fructose 1,6-bisphosphate aldolase class II Mycobacterium tuberculosis
4.1.2.13 10
-
 fructose 1-phosphate
-
 Mycolicibacterium smegmatis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.13 K+ no effect Mycolicibacterium smegmatis
4.1.2.13 K+ stimulates Mycobacterium tuberculosis
4.1.2.13 Mg2+ no effect Mycolicibacterium smegmatis
4.1.2.13 Zn2+ no effect Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.13 40000
-
 4 * 40000, SDS-PAGE Mycolicibacterium smegmatis
4.1.2.13 158000
-
 gel filtration Mycolicibacterium smegmatis

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.13 Mycobacterium tuberculosis
-
 H37Rv, NCTC, 7416: aldolase class I
-
4.1.2.13 Mycobacterium tuberculosis
-
 NCTC, 7416: aldolase class II
-
4.1.2.13 Mycolicibacterium smegmatis
-
 CDC No. 46
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.13
-
 Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.13 additional information
-
-
 Mycolicibacterium smegmatis
4.1.2.13 additional information
-
-
 Mycobacterium tuberculosis

Storage Stability

EC Number Storage Stability Organism
4.1.2.13 -20°C, stable for 3 weeks Mycolicibacterium smegmatis
4.1.2.13 -20°C, stable for 4 weeks Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.13 D-fructose 1,6-bisphosphate
-
 Mycolicibacterium smegmatis glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.13 D-fructose 1,6-bisphosphate
-
 Mycobacterium tuberculosis glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.13 D-Fructose 1-phosphate
-
 Mycolicibacterium smegmatis Glycerone phosphate + D-glyceraldehyde
-
 ?
4.1.2.13 D-Fructose 1-phosphate no activity Mycobacterium tuberculosis Glycerone phosphate + D-glyceraldehyde
-
 ?
4.1.2.13 Glycerone phosphate + D-glyceraldehyde 3-phosphate aldol synthesizing activity is only 1.5% of fructose 1,6-diphosphate cleavage Mycolicibacterium smegmatis D-Fructose 1,6-bisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.13 tetramer 4 * 40000, SDS-PAGE Mycolicibacterium smegmatis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.13 7.9
-
-
 Mycolicibacterium smegmatis
4.1.2.13 8.6
-
 aldolase class II Mycobacterium tuberculosis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.2.13 5
-
 30 min, 90% loss of activity Mycobacterium tuberculosis