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Literature summary extracted from

  • Laiz, L.; Liras, P.; Castro, J.M.; Martin, J.F.
    Purification and characterization of the isopenicillin-N epimerase from Nocardia lactamdurans (1990), J. Gen. Microbiol., 136, 663-671.
No PubMed abstract available

General Stability

EC Number General Stability Organism
5.1.1.17 unstable enzyme, pyridoxal 5'-phosphate stabilizes Amycolatopsis lactamdurans

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.1.1.17 4-chloromercuribenzoate 0.1 mM, 36% inhibition Amycolatopsis lactamdurans
5.1.1.17 ammonium sulfate 100 mM, 47% inhibition Amycolatopsis lactamdurans
5.1.1.17 Cu2+ 1 mM, complete inhibition Amycolatopsis lactamdurans
5.1.1.17 Fe2+ 0.135 mM, complete inhibition Amycolatopsis lactamdurans
5.1.1.17 Hg2+ 1 mM, complete inhibition Amycolatopsis lactamdurans
5.1.1.17 I- 1 mM, 27% inhibition Amycolatopsis lactamdurans
5.1.1.17 iodoacetamide 0.1 mM; 40% inhibition Amycolatopsis lactamdurans
5.1.1.17 N-ethylmaleimide 0.1 mM; 33% inhibition Amycolatopsis lactamdurans
5.1.1.17 Zn2+ 1 mM, 50% inhibition Amycolatopsis lactamdurans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.1.1.17 0.27
-
 isopenicillin N
-
 Amycolatopsis lactamdurans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.1.17 59000
-
 gel filtration Amycolatopsis lactamdurans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.1.1.17 isopenicillin N Amycolatopsis lactamdurans part of the cephalosporin pathway penicillin N
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.1.1.17 Amycolatopsis lactamdurans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.1.17 protamine sulfate, ammonium sulfate, Sephadex G-75, Mono Q Amycolatopsis lactamdurans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.1.1.17 0.000727
-
-
 Amycolatopsis lactamdurans

Storage Stability

EC Number Storage Stability Organism
5.1.1.17 -70°C, 0.05 mM pyridoxal 5'-phosphate, 17 d, no loss of activity Amycolatopsis lactamdurans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.1.1.17 isopenicillin
-
 Amycolatopsis lactamdurans penicillin N
-
 r
5.1.1.17 isopenicillin N part of the cephalosporin pathway Amycolatopsis lactamdurans penicillin N
-
 r

Subunits

EC Number Subunits Comment Organism
5.1.1.17 monomer 1 * 59000, SDS-PAGE Amycolatopsis lactamdurans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.1.1.17 25
-
 slightly lower activity at 20 and 30°C Amycolatopsis lactamdurans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.1.1.17 5 45 reduced activity below 20°C or above 30°C Amycolatopsis lactamdurans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.1.1.17 20 40 stable for 2 h Amycolatopsis lactamdurans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1.1.17 7
-
-
 Amycolatopsis lactamdurans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.1.1.17 6.5 8 strong decrease in activity above pH 8.0 and below pH 6.5 Amycolatopsis lactamdurans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.1.1.17 4.5 9
-
 Amycolatopsis lactamdurans

Cofactor

EC Number Cofactor Comment Organism Structure
5.1.1.17 pyridoxal 5'-phosphate stabilizes enzyme during purification, may be tightly bound to the enzyme Amycolatopsis lactamdurans