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Literature summary extracted from

  • Kawaide, H.; Imai, R.; Sassa, T.; Kamiya, Y.
    ent-Kaurene synthetase from the fungus Phaeosphaeria sp. L487. cDNA isolation, characterization, and bacterial expression of a bifunctional diterpene cyclase in fungal gibberellin biosynthesis (1997), J. Biol. Chem., 272, 21706-21712.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.19 expressed in Escherichia coli JM109 as glutathione-S-transferase fusion protein, enzyme shows EC 4.2.3.19 and EC 5.5.1.13 activity Phaeosphaeria sp.
5.5.1.13 expression in Escherichia coli of a recombinant GST fused enzyme Phaeosphaeria sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.19 2'-isopropyl-4'(trimethylammonium chloride)-5'-methylphenyl piperidine-1-carboxylate Amo-1618 Phaeosphaeria sp.
4.2.3.19 EDTA
-
 Phaeosphaeria sp.
5.5.1.13 2'-isopropyl-4'-(trimethylammonium chloride)-5'-methylphenyl-piperidine-1-carboxylate AMO-1618, a quaternary ammonium salt, leads to total enzyme inhibition at 0.001 mM Phaeosphaeria sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.19 Mg2+ required Phaeosphaeria sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.19 132000
-
 SDS-PAGE, cloned fusion protein Phaeosphaeria sp.
5.5.1.13 106000
-
 calculated from amino acid sequence Phaeosphaeria sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.19 ent-copalyl diphosphate Phaeosphaeria sp. involved in biosynthesis of gibberellins ent-kaurene + diphosphate
-
 ?
4.2.3.19 ent-copalyl diphosphate Phaeosphaeria sp. L487 involved in biosynthesis of gibberellins ent-kaurene + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.19 Phaeosphaeria sp. O13284
-
-
4.2.3.19 Phaeosphaeria sp. L487 O13284
-
-
5.5.1.13 Phaeosphaeria sp. O13284 from mycelia
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.5.1.13 recombinant enzyme, using Sepharose 4B glutathione affinity resin and SDS PAGE electrophoresis Phaeosphaeria sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.19 ent-copalyl diphosphate
-
 Phaeosphaeria sp. ent-kaurene + diphosphate
-
 ?
4.2.3.19 ent-copalyl diphosphate involved in biosynthesis of gibberellins Phaeosphaeria sp. ent-kaurene + diphosphate
-
 ?
4.2.3.19 ent-copalyl diphosphate
-
 Phaeosphaeria sp. L487 ent-kaurene + diphosphate
-
 ?
4.2.3.19 ent-copalyl diphosphate involved in biosynthesis of gibberellins Phaeosphaeria sp. L487 ent-kaurene + diphosphate
-
 ?
5.5.1.13 copalyl diphosphate bifunctional enzyme Phaeosphaeria sp. ent-kaurene + diphosphate
-
 ?
5.5.1.13 geranylgeranyl diphosphate
-
 Phaeosphaeria sp. copalyl diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.19 additional information part of bifunctional enzyme catalyzing the two step reaction from trans-geranylgeranyl-diphosphate to ent-kaurene, first step is catalyzed by EC 5.5.1.13 (ent-kaurene synthase A) Phaeosphaeria sp.