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Literature summary extracted from

  • Lorenz, W.; Inglese, J.; Palczewski, K.; Onorato, J.J.; Caron, M.G.; Lefkowitz, R.J.
    The receptor kinase family: primary structure of rhodopsin kinase reveals similarities to the beta-adrenergic receptor kinase (1991), Proc. Natl. Acad. Sci. USA, 88, 8715-8719.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.14
-
 Bos taurus
2.7.11.14 cDNA encoding enzyme is cloned and expressed in COS-7 cells, sequence of the 561 amino acids protein Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.14 membrane rod membranes Bos taurus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.14 Mg2+ requirement Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.14 additional information
-
 amino acid sequence shows high degree of homology to beta-adrenergic receptor kinase primary structure Bos taurus
2.7.11.14 62900
-
 x * 62900, calculated from the amino acid sequence Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.14 ATP + rhodopsin Bos taurus light-dependent deactivation of rhodopsin involves receptor phosphorylation that is mediated by the highly specific protein kinases rhodopsin kinase ADP + phosphorylated rhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin Bos taurus
-
 ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin Bos taurus light-dependent initiating of deactivation of rhodopsin ADP + phosphorhodopsin
-
 ?
2.7.11.14 additional information Bos taurus RK phosphorylates other color opsins in vivo ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.14 Bos taurus
-
-
-
2.7.11.14 Bos taurus P28327
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.14 additional information
-
 Bos taurus
2.7.11.14 additional information the primary structure of enzyme suggests several posttranslational modifications, e.g. myristoylation, phosphorylation, isoprenylation sites Bos taurus
2.7.11.14 phosphoprotein enzyme is autophosphorylated Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.14 1000fold Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.14 eye retina Bos taurus
-
2.7.11.14 pineal gland contains RK mRNA Bos taurus
-
2.7.11.14 retina rod cell outer segment Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.14 ATP + rhodopsin light-dependent deactivation of rhodopsin involves receptor phosphorylation that is mediated by the highly specific protein kinases rhodopsin kinase Bos taurus ADP + phosphorylated rhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin
-
 Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin domain structure, catalytic domain of 270 amino acids in the center of the sequence Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin highly specific for rhodopsin Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin light-dependent phosphorylation Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin highly specific for photobleached rhodopsin Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin phosphorylates serine and threonine residues in the carboxy-terminal region of opsin peptide Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 ATP + rhodopsin light-dependent initiating of deactivation of rhodopsin Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.14 additional information RK phosphorylates other color opsins in vivo Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.14 ?
-
 Bos taurus
2.7.11.14 ? x * 62900, calculated from the amino acid sequence Bos taurus

Synonyms

EC Number Synonyms Comment Organism
2.7.11.14 rhodopsin kinase
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.14 25
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.14 7.5
-
 assay at Bos taurus