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Literature summary extracted from

  • Benovic, J.L.; DeBlasi, A.; Stone, W.C.; Caron, M.G.; Lefkowitz, R.J.
    Beta-adrenergic receptor kinase: primary structure delineates a multigene family (1989), Science, 246, 235-240.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.15 isoproterenol requirement, beta-agonist, beta-adrenergic receptor as substrate Bos taurus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.15 cDNA encoding beta-ARK is cloned from brain, sequenced and expressed in COS-7 cells, cDNA encodes a 689 amino acids protein Bos taurus
2.7.11.15 expression in COS-7 cells Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.15 Mg2+ requirement Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.15 79700
-
 x * 79700, calculated from the amino acid sequence Bos taurus
2.7.11.15 80000
-
 x * 80000, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.15 ATP + beta-adrenergic receptor Bos taurus agonist-occupied form of the receptor ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor Bos taurus involved in homologous desensitization of beta-adrenergic receptor ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 additional information Bos taurus enzyme is important in mediating rapid agonist-specific desensitization ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.15 Bos taurus
-
-
-
2.7.11.15 Bos taurus P21146
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.15 about 20000fold, from brain Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.15 brain
-
 Bos taurus
-
2.7.11.15 brain cerebral cortex Bos taurus
-
2.7.11.15 brain highest beta-ARK mRNA concentrations in brain and spleen, brain: highest levels in cerebral cortex and cerebellum, with significant lower levels in basal ganglia, brain stem, pituitary and hypothalamus Bos taurus
-
2.7.11.15 cerebellum brain: highest beta-ARK mRNA concentrations in cerebral cortex and cerebellum Bos taurus
-
2.7.11.15 cerebral cortex brain: highest beta-ARK mRNA concentrations in cerebral cortex and cerebellum Bos taurus
-
2.7.11.15 heart about 40% of beta-ARK mRNA concentration in brain Bos taurus
-
2.7.11.15 kidney about 20% of beta-ARK mRNA concentration in brain Bos taurus
-
2.7.11.15 lung about 40% of beta-ARK mRNA concentration in brain Bos taurus
-
2.7.11.15 additional information tissue distribution Bos taurus
-
2.7.11.15 additional information not in liver, muscle and adrenal gland Bos taurus
-
2.7.11.15 ovary about 20% of beta-ARK mRNA concentration in brain Bos taurus
-
2.7.11.15 spleen highest beta-ARK mRNA concentrations in brain and spleen Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.15 ATP + beta-adrenergic receptor beta2-adrenergic receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor specifically phosphorylates the agonist-occupied form of the receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor beta-AR from hamster lung Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor beta-AR is a much better substrate than rhodopsin Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor agonist-occupied form of the receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + beta-adrenergic receptor involved in homologous desensitization of beta-adrenergic receptor Bos taurus ADP + phospho-beta-adrenergic receptor
-
 ?
2.7.11.15 ATP + rhodopsin beta-AR is a much better substrate than rhodopsin Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.15 ATP + rhodopsin light-dependent, actual substrate: light-bleached rhodopsin Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.15 ATP + rhodopsin in form of bovine rod outer segments Bos taurus ADP + phosphorhodopsin
-
 ?
2.7.11.15 additional information domain structure Bos taurus ?
-
 ?
2.7.11.15 additional information enzyme is important in mediating rapid agonist-specific desensitization Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.15 ? x * 80000, SDS-PAGE Bos taurus
2.7.11.15 ? x * 79700, calculated from the amino acid sequence Bos taurus

Synonyms

EC Number Synonyms Comment Organism
2.7.11.15 beta-adrenergic receptor kinase
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.15 30
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.15 7.5
-
 assay at Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.15 ATP
-
 Bos taurus