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Literature summary extracted from

  • Holtz, J.; Klämbt, D.
    tRNA isopentenyltransferase from Zea mays L. Characterization of the isopentenylation reaction of tRNA, oligo (A) and other nucleic acids (1978), Hoppe-Seyler's Z. Physiol. Chem., 359, 89-101.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.75 diphosphate
-
 Zea mays
2.5.1.75 EDTA
-
 Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.75 additional information
-
 additional information KM of substrates and tRNA from various species Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.75 Mg2+ 3-5 mM Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.75 60000
-
 gel filtration, SDS-PAGE Zea mays

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.75 Zea mays
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.75 kernel
-
 Zea mays
-
2.5.1.75 leaf
-
 Zea mays
-
2.5.1.75 root tip
-
 Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.75 additional information
-
-
 Zea mays

Storage Stability

EC Number Storage Stability Organism
2.5.1.75 -18°C, 0.59 mg bovine serum albumin/ml, 50% glycerol, purified enzyme loses 80% of activity after 14 days Zea mays
2.5.1.75 -18°C, 50% glycerol, partially purified enzyme stable for 3 months Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.75 dimethylallyl diphosphate + adenine37 in tRNA no reaction with DELTA3-isopentenyl diphosphate Zea mays diphosphate + N6-dimethylallyladenine37 in tRNA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.75 monomer 1 * 60000, SDS-PAGE Zea mays

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.75 37
-
-
 Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.75 7.8
-
-
 Zea mays