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Literature summary extracted from

  • Jones, R.M.; Jordan, P.M.
    Purification and properties of porphobilinogen deaminase from Arabidopsis thaliana (1994), Biochem. J., 299, 895-902.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.61 2-bromoporphobilinogen
-
 Arabidopsis thaliana
2.5.1.61 Ag2+ 1 mM AgCl2, 90% inhibition Arabidopsis thaliana
2.5.1.61 Cd2+ 0.1 mM CdCl2, 55% inhibition Arabidopsis thaliana
2.5.1.61 Co2+ 0.1 mM CoCl2, 95% inhibition Arabidopsis thaliana
2.5.1.61 Cu2+ 1 mM CuSO4, 66% inhibition Arabidopsis thaliana
2.5.1.61 Hg2+ 0.1 mm HgCl2, 76% inhibition Arabidopsis thaliana
2.5.1.61 hydroxylamine
-
 Arabidopsis thaliana
2.5.1.61 K+ at high concentrations Arabidopsis thaliana
2.5.1.61 additional information
-
 Arabidopsis thaliana
2.5.1.61 Na+ at high concentrations Arabidopsis thaliana
2.5.1.61 NH4+
-
 Arabidopsis thaliana
2.5.1.61 Zn2+ 1 mM ZnCl2, 78% inhibition Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.61 0.017
-
 porphobilinogen
-
 Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.61 31000
-
 gel filtration Arabidopsis thaliana
2.5.1.61 35000
-
 1 * 35000, SDS-PAGE Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.61 porphobilinogen Arabidopsis thaliana
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.61 Arabidopsis thaliana
-
 Columbia
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.61
-
 Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.61 leaf
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.61 additional information
-
-
 Arabidopsis thaliana
2.5.1.61 0.073
-
-
 Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.61 4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Arabidopsis thaliana uroporphyrinogen III + 4 NH3
-
 ?
2.5.1.61 4 porphobilinogen + H2O
-
 Arabidopsis thaliana hydroxylmethylbilane + 4 NH3 1-hydroxymethylbilane, highly unstable ?
2.5.1.61 porphobilinogen
-
 Arabidopsis thaliana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.61 monomer 1 * 35000, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.5.1.61 porphobilinogen deaminase
-
 Arabidopsis thaliana

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.61 70
-
 10 min, heat-stable up to 70°C Arabidopsis thaliana
2.5.1.61 80
-
 10 min, 40% loss of activity Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.61 7.7 8.5
-
 Arabidopsis thaliana
2.5.1.61 8
-
-
 Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.61 6 10 pH 6.0: about 15% of maximal activity, pH 10.0: 25% of maximal activity Arabidopsis thaliana