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Literature summary extracted from

  • Davies, R.C.; Neuberger, A.
    Polypyrroles formed from porphobilinogen and amines by uroporphyrinogen synthetase of Rhodopseudomonas spheroides (1973), Biochem. J., 133, 471-492.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.61 aniline
-
 Cereibacter sphaeroides
2.5.1.61 Ca2+
-
 Cereibacter sphaeroides
2.5.1.61 hydrazine
-
 Cereibacter sphaeroides
2.5.1.61 hydroxylamine
-
 Cereibacter sphaeroides
2.5.1.61 methoxyamine
-
 Cereibacter sphaeroides
2.5.1.61 methylamine
-
 Cereibacter sphaeroides
2.5.1.61 Mg2+
-
 Cereibacter sphaeroides
2.5.1.61 additional information mechanism of inhibition Cereibacter sphaeroides
2.5.1.61 additional information
-
 Triticum aestivum
2.5.1.61 NH4+
-
 Cereibacter sphaeroides
2.5.1.61 opsopyrroledicarboxylic acid 1 mM, 40% inhibition Cereibacter sphaeroides
2.5.1.61 opsopyrroledicarboxylic acid
-
 Triticum aestivum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.61 0.04
-
 porphobilinogen
-
 Cereibacter sphaeroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.61 35000
-
 gel filtration Cereibacter sphaeroides
2.5.1.61 36000
-
 1 * 36000, SDS-PAGE in the presence of beta-mercaptoethanol Cereibacter sphaeroides
2.5.1.61 36000
-
 1 * 36000, SDS-PAGE in the presence and in the absence of 2-mercaptoethanol Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.61 porphobilinogen Bos taurus
-
 ?
-
 ?
2.5.1.61 porphobilinogen Triticum aestivum
-
 ?
-
 ?
2.5.1.61 porphobilinogen Cereibacter sphaeroides
-
 ?
-
 ?
2.5.1.61 porphobilinogen Spinacia oleracea
-
 ?
-
 ?
2.5.1.61 porphobilinogen Glycine max
-
 ?
-
 ?
2.5.1.61 porphobilinogen Cereibacter sphaeroides NCIB 8253
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.61 Bos taurus
-
-
-
2.5.1.61 Cereibacter sphaeroides
-
 N.C.I.B. 8253
-
2.5.1.61 Cereibacter sphaeroides NCIB 8253
-
 N.C.I.B. 8253
-
2.5.1.61 Glycine max
-
 soya-bean
-
2.5.1.61 Spinacia oleracea
-
 spinach
-
2.5.1.61 Triticum aestivum
-
 wheat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.61
-
 Cereibacter sphaeroides

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.61 callus
-
 Bos taurus
-
2.5.1.61 callus
-
 Glycine max
-
2.5.1.61 germ
-
 Triticum aestivum
-
2.5.1.61 leaf
-
 Spinacia oleracea
-
2.5.1.61 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.61 additional information
-
-
 Cereibacter sphaeroides
2.5.1.61 0.45
-
-
 Cereibacter sphaeroides

Storage Stability

EC Number Storage Stability Organism
2.5.1.61 -20°C, stable for over 1 year Cereibacter sphaeroides
2.5.1.61 0°C, stable in solution at pH 7.5 and 9.5 for 3 weeks Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.61 4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Bos taurus uroporphyrinogen III + 4 NH3
-
 ?
2.5.1.61 4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Triticum aestivum uroporphyrinogen III + 4 NH3
-
 ?
2.5.1.61 4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Cereibacter sphaeroides uroporphyrinogen III + 4 NH3
-
 ?
2.5.1.61 4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Spinacia oleracea uroporphyrinogen III + 4 NH3
-
 ?
2.5.1.61 4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Glycine max uroporphyrinogen III + 4 NH3
-
 ?
2.5.1.61 4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Cereibacter sphaeroides NCIB 8253 uroporphyrinogen III + 4 NH3
-
 ?
2.5.1.61 4 porphobilinogen + H2O
-
 Bos taurus hydroxylmethylbilane + 4 NH3
-
 ?
2.5.1.61 4 porphobilinogen + H2O
-
 Triticum aestivum hydroxylmethylbilane + 4 NH3
-
 ?
2.5.1.61 4 porphobilinogen + H2O
-
 Spinacia oleracea hydroxylmethylbilane + 4 NH3
-
 ?
2.5.1.61 4 porphobilinogen + H2O
-
 Glycine max hydroxylmethylbilane + 4 NH3
-
 ?
2.5.1.61 4 porphobilinogen + H2O
-
 Cereibacter sphaeroides hydroxylmethylbilane + 4 NH3 at pH 8.2 ?
2.5.1.61 4 porphobilinogen + H2O
-
 Cereibacter sphaeroides hydroxylmethylbilane + 4 NH3 uroporphyrinogen I ?
2.5.1.61 4 porphobilinogen + H2O
-
 Cereibacter sphaeroides NCIB 8253 hydroxylmethylbilane + 4 NH3 at pH 8.2 ?
2.5.1.61 4 porphobilinogen + H2O
-
 Cereibacter sphaeroides NCIB 8253 hydroxylmethylbilane + 4 NH3 uroporphyrinogen I ?
2.5.1.61 porphobilinogen
-
 Bos taurus ?
-
 ?
2.5.1.61 porphobilinogen
-
 Triticum aestivum ?
-
 ?
2.5.1.61 porphobilinogen
-
 Cereibacter sphaeroides ?
-
 ?
2.5.1.61 porphobilinogen
-
 Spinacia oleracea ?
-
 ?
2.5.1.61 porphobilinogen
-
 Glycine max ?
-
 ?
2.5.1.61 porphobilinogen
-
 Cereibacter sphaeroides NCIB 8253 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.61 monomer 1 * 36000, SDS-PAGE in the presence of beta-mercaptoethanol Cereibacter sphaeroides
2.5.1.61 monomer 1 * 36000, SDS-PAGE in the presence and in the absence of 2-mercaptoethanol Cereibacter sphaeroides

Synonyms

EC Number Synonyms Comment Organism
2.5.1.61 porphobilinogen deaminase
-
 Cereibacter sphaeroides
2.5.1.61 uroporphyrinogen I synthetase
-
 Cereibacter sphaeroides
2.5.1.61 uroporphyrinogen I synthetase
-
 Spinacia oleracea
2.5.1.61 uroporphyrinogen synthetase
-
 Cereibacter sphaeroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.61 37
-
 assay at Cereibacter sphaeroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.61 7.8 8 0.1-0.4 mM porphobilinogen Cereibacter sphaeroides

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.61 6.5 9.2 50% of maximal activity at pH 6.5 and pH 9.2 Cereibacter sphaeroides