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Literature summary extracted from

  • Hommes, B.G.; Akiyosh, D.E.; Morris, R.O.
    Assay and partial purification of the cytokinin biosynthetic enzyme dimethylallylpyrophosphate:5'-AMP transferase (1985), Methods Enzymol., 110, 340-347.
No PubMed abstract available

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.27 Nicotiana tabacum
-
 tobacco, crown gall tumor line 15055/01
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.27 partial Nicotiana tabacum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.27 crown gall crown gall tumor line 15055/01 Nicotiana tabacum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.27 additional information
-
 assay principle Nicotiana tabacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.27 dimethylallyl diphosphate + AMP
-
 Nicotiana tabacum diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
 ?
2.5.1.27 additional information strict requirement for AMP, no substrate: adenine, adenosine Nicotiana tabacum ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.27 AMP strict requirement Nicotiana tabacum