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Literature summary extracted from

  • Sindhu, R.K.; Cohen, S.S.
    Propylaminetransferases in chinese cabbage leaves (1984), Plant Physiol., 74, 645-649.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.5.1.16 dialysis against glycine-NaOH buffer, pH 8.8 inactivates Brassica rapa subsp. pekinensis
2.5.1.16 DTT and/or EDTA do not stabilize Brassica rapa subsp. pekinensis
2.5.1.16 very labile in crude extracts Brassica rapa subsp. pekinensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.16 5'-deoxy-5'-methylthioadenosine weak Brassica rapa subsp. pekinensis
2.5.1.16 5'-deoxy-5'-methylthiotubercidine weak Brassica rapa subsp. pekinensis
2.5.1.16 Cyclohexylamine strong Brassica rapa subsp. pekinensis
2.5.1.16 S-adenosyl-(5')-3-methylthio-1-propylamine at concentrations above 0.05 mM Brassica rapa subsp. pekinensis
2.5.1.16 S-Adenosyl-1,8-diamino-3-thiooctane transition-state analogue, potent inhibition Brassica rapa subsp. pekinensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.16 0.0067
-
 S-adenosyl-3-methylthio-1-propylamine pH 8.8, 37ºC, crude extracts Brassica rapa subsp. pekinensis
2.5.1.16 0.0067
-
 S-adenosyl-3-methylthio-1-propylamine pH 7.4, 37ºC, partially purified enzyme Brassica rapa subsp. pekinensis
2.5.1.16 0.032
-
 1,4-diaminobutane pH 8.8, 37ºC, crude extracts Brassica rapa subsp. pekinensis
2.5.1.16 0.032
-
 1,4-diaminobutane pH 7.4, 37ºC, partially purified enzyme Brassica rapa subsp. pekinensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.16 81000
-
 gel filtration Brassica rapa subsp. pekinensis

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.16 Brassica rapa subsp. pekinensis
-
 Chinese cabbage, var Pak Choy
-
2.5.1.16 Spinacia oleracea
-
 spinach
-
2.5.1.22 Brassica rapa subsp. pekinensis
-
 Chinese cabbage, var. Pak Choy
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.16 ammonium sulfate fractionation, acetone precipitation and chromatography on Sephadex G-100, 160fold purified Brassica rapa subsp. pekinensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.16 leaf
-
 Spinacia oleracea
-
2.5.1.16 leaf
-
 Brassica rapa subsp. pekinensis
-
2.5.1.22 leaf
-
 Brassica rapa subsp. pekinensis
-

Storage Stability

EC Number Storage Stability Organism
2.5.1.16 -20ºC, 30% loss of activity in 1 month, after final filtration through Sephadex Brassica rapa subsp. pekinensis
2.5.1.16 -20ºC, stable for at least 3 months after acetone precipitation Brassica rapa subsp. pekinensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.16 S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane the reaction is linear from 1 to 10 nmol of spermidine Brassica rapa subsp. pekinensis 5'-methylthioadenosine + spermidine
-
 ?
2.5.1.22 S-adenosylmethioninamine + spermidine
-
 Brassica rapa subsp. pekinensis 5'-methylthioadenosine + spermine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.16 37
-
 assay at Spinacia oleracea
2.5.1.16 37
-
 assay at Brassica rapa subsp. pekinensis
2.5.1.22 37
-
-
 Brassica rapa subsp. pekinensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.16 8.8
-
 in Gly-NaOH buffer Brassica rapa subsp. pekinensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.16 additional information
-
 rapidly inactivated at pH more than 9.0 in Gly-NaOH buffer. The activity of the enzyme is generally lower in Tris buffer than in Gly-NaOH buffer Brassica rapa subsp. pekinensis