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Literature summary extracted from

  • Raina, A.; Pajula, R.L.; Eloranta, T.
    Purification of spermidine aminopropyltransferase (spermine synthase) from bovine brain (1983), Methods Enzymol., 94, 276-279.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.22 5'-methylthioadenosine strong Bos taurus
2.5.1.22 additional information not inhibitory: decarboxylated adenosylmethionine Bos taurus
2.5.1.22 N-ethylmaleimide in the absence of sulfhydryl compounds Bos taurus
2.5.1.22 PCMB in the absence of sulfhydryl compounds Bos taurus
2.5.1.22 putrescine weak Bos taurus
2.5.1.22 spermine product inhibition, weak Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.22 0.0006
-
 S-adenosylmethioninamine
-
 Bos taurus
2.5.1.22 0.006
-
 spermidine
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.22 45000
-
 2 * 45000, SDS-PAGE Bos taurus
2.5.1.22 88000 90000 brain, gel filtration, sedimentation equilibrium centrifugation Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.22 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.22
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.22 brain
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.22 0.396
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.5.1.22 0°C, in buffer containing 0.3 M NaCl and 1 mM spermidine, at least 6 months Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.22 additional information S-adenosylmethionine cannot act as aminopropyl donor Bos taurus ?
-
 ?
2.5.1.22 S-adenosylmethioninamine + spermidine strictly specific for spermidine Bos taurus 5'-methylthioadenosine + spermine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.22 dimer 2 * 45000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.22 37
-
-
 Bos taurus