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Literature summary extracted from

  • Pajula, R.L.; Raina, A.; Eloranta, T.
    Polyamine synthesis in mammalian tissues. Isolation and characterization of spermine synthase from bovine brain (1979), Eur. J. Biochem., 101, 619-626.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.22 5'-methylthioadenosine strong Bos taurus
2.5.1.22 additional information no inhibition by erythro-9-(2-hydroxy-3-nonyl)adenine, methylglyoxal-bis-(guanylhydrazone), aminoguanidine, guanethidine, L-canaline, MgCl2, KCl, diamines, polyamines, polyamine derivatives Bos taurus
2.5.1.22 N-ethylmaleimide in the absence of sulfhydryl compounds Bos taurus
2.5.1.22 PCMB in the absence of sulfhydryl compounds Bos taurus
2.5.1.22 putrescine
-
 Bos taurus
2.5.1.22 S-adenosyl-L-homocysteine not Bos taurus
2.5.1.22 S-adenosyl-L-methionine not Bos taurus
2.5.1.22 spermine product inhibition, weak Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.22 0.0006
-
 S-adenosylmethioninamine
-
 Bos taurus
2.5.1.22 0.006
-
 spermidine
-
 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.22 additional information no metal ion requirement Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.22 45000
-
 2 * 45000, SDS-PAGE Bos taurus
2.5.1.22 88000 90000 brain, gel filtration, sedimentation equilibrium centrifugation Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.22 S-adenosylmethioninamine + spermidine Bos taurus involved in polyamine biosynthetic pathway 5'-methylthioadenosine + spermine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.22 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.22 spermine-Sepharose affinity chromatography Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.22 brain
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.22 0.396
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.22 additional information overview Bos taurus ?
-
 ?
2.5.1.22 S-adenosylmethioninamine + spermidine strictly specific for spermidine Bos taurus 5'-methylthioadenosine + spermine
-
 ?
2.5.1.22 S-adenosylmethioninamine + spermidine involved in polyamine biosynthetic pathway Bos taurus 5'-methylthioadenosine + spermine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.22 dimer 2 * 45000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.22 37
-
-
 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.22 additional information
-
 pI: 5.1, brain Bos taurus
2.5.1.22 7 8 broad Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.22 additional information no cofactor requirement Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.22 0.0003
-
 5'-methylthioadenosine
-
 Bos taurus
2.5.1.22 0.0017
-
 putrescine
-
 Bos taurus