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Literature summary extracted from

  • Lowery, R.G.; Ludden, P.W.
    Purification and properties of dinitrogenase reductase ADP-ribosyltransferase from the photosynthetic bacterium Rhodospirillum rubrum (1988), J. Biol. Chem., 263, 16714-16719.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.2.37 ADP and NaCl stabilize during purification and storage Rhodospirillum rubrum
2.4.2.37 desalting procedures during purification inactivate Rhodospirillum rubrum
2.4.2.37 DTT stabilizes during purification Rhodospirillum rubrum
2.4.2.37 extremely unstable in crude extract or partially purified preparation Rhodospirillum rubrum
2.4.2.37 one freeze-thawing cycle leads to 50% loss of activity, ADP stabilizes Rhodospirillum rubrum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.37 3-aminobenzamide
-
Rhodospirillum rubrum
2.4.2.37 KCl reversible Rhodospirillum rubrum
2.4.2.37 NaBr strong Rhodospirillum rubrum
2.4.2.37 NaCl reversible Rhodospirillum rubrum
2.4.2.37 NADH
-
Rhodospirillum rubrum
2.4.2.37 NADP+
-
Rhodospirillum rubrum
2.4.2.37 nicotinamide
-
Rhodospirillum rubrum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.37 2
-
NAD+
-
Rhodospirillum rubrum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.2.37 soluble
-
Rhodospirillum rubrum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.37 29000
-
1 * 29000, SDS-PAGE Rhodospirillum rubrum
2.4.2.37 30900
-
gel filtration Rhodospirillum rubrum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.37 NAD+ + [dinitrogen reductase] Rhodospirillum rubrum involved in regulation of nitrogenase EC 1.18.6.1 activity ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.37 Rhodospirillum rubrum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.37 to near homogeneity Rhodospirillum rubrum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.37 0.077
-
-
Rhodospirillum rubrum

Storage Stability

EC Number Storage Stability Organism
2.4.2.37 -80°C, in liquid N2 Rhodospirillum rubrum
2.4.2.37 0°C, in 0.2 M NaCl and 1 mM ADP, 18 h Rhodospirillum rubrum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.37 etheno-NAD+ + [dinitrogen reductase]
-
Rhodospirillum rubrum ?
-
?
2.4.2.37 NAD+ + [dinitrogen reductase] high specificity for acceptor substrate, the only acceptor is dinitrogen reductase Rhodospirillum rubrum nicotinamide + ADP-D-ribosyl-[dinitrogen reductase]
-
?
2.4.2.37 NAD+ + [dinitrogen reductase] involved in regulation of nitrogenase EC 1.18.6.1 activity Rhodospirillum rubrum ?
-
?
2.4.2.37 nicotinamide guanine dinucleotide + [dinitrogen reductase]
-
Rhodospirillum rubrum ?
-
?
2.4.2.37 nicotinamide hypoxanthine dinucleotide + [dinitrogen reductase]
-
Rhodospirillum rubrum ?
-
?

Subunits

EC Number Subunits Comment Organism
2.4.2.37 monomer 1 * 29000, SDS-PAGE Rhodospirillum rubrum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.37 30
-
assay at Rhodospirillum rubrum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.37 7
-
-
Rhodospirillum rubrum